Next-Generation Sequencing in Equine Genomics

Jessica L Petersen; Stephen J Coleman

doi:10.1016/j.cveq.2020.03.002

Next-Generation Sequencing in Equine Genomics

Vet Clin North Am Equine Pract. 2020 Aug;36(2):195-209. doi: 10.1016/j.cveq.2020.03.002.

Authors

Jessica L Petersen¹, Stephen J Coleman²

Affiliations

¹ Department of Animal Science, University of Nebraska-Lincoln, ANSC A218g, 3940 Fair Street, Lincoln, NE 68583-0908, USA.
² Department of Animal Sciences, Colorado State University, 1171 Campus Delivery, Fort Collins, CO 80523-1171, USA. Electronic address: stephen.coleman@colostate.edu.

PMID: 32654781
DOI: 10.1016/j.cveq.2020.03.002

Abstract

The sequencing and assembly of a reference genome for the horse has been revolutionary for investigation of horse health and performance. Next-generation sequencing (NGS) methods represent a second revolution in equine genomics. Researchers can align and compare DNA and RNA sequencing data to the reference genome to explore variation that may contribute or be attributed to disease. NGS has also facilitated the translation of research discovery to clinically relevant applications. This article discusses the history and development of NGS, details some of the available sequencing platforms, and describes currently available applications in the context of both discovery and clinical settings.

Keywords: Equine; Genetic variation; Genomics; RNA sequencing; Transcriptome.

Publication types

Review

MeSH terms

Animals
Genomics / methods
High-Throughput Nucleotide Sequencing / methods
High-Throughput Nucleotide Sequencing / veterinary*
Horse Diseases / genetics
Horses / genetics*
Sequence Analysis, DNA / methods
Sequence Analysis, DNA / veterinary*