Low phosphorus supply constrains plant responses to elevated CO2 : A meta-analysis

Abstract

Phosphorus (P) is an essential macro-nutrient required for plant metabolism and growth. Low P availability could potentially limit plant responses to elevated carbon dioxide (eCO₂ ), but consensus has yet to be reached on the extent of this limitation. Here, based on data from experiments that manipulated both CO₂ and P for young individuals of woody and non-woody species, we present a meta-analysis of P limitation impacts on plant growth, physiological, and morphological response to eCO₂ . We show that low P availability attenuated plant photosynthetic response to eCO₂ by approximately one-quarter, leading to a reduced, but still positive photosynthetic response to eCO₂ compared to those under high P availability. Furthermore, low P limited plant aboveground, belowground, and total biomass responses to eCO₂ , by 14.7%, 14.3%, and 12.4%, respectively, equivalent to an approximate halving of the eCO₂ responses observed under high P availability. In comparison, low P availability did not significantly alter the eCO₂ -induced changes in plant tissue nutrient concentration, suggesting tissue nutrient flexibility is an important mechanism allowing biomass response to eCO₂ under low P availability. Low P significantly reduced the eCO₂ -induced increase in leaf area by 14.3%, mirroring the aboveground biomass response, but low P did not affect the eCO₂ -induced increase in root length. Woody plants exhibited stronger attenuation effect of low P on aboveground biomass response to eCO₂ than non-woody plants, while plants with different mycorrhizal associations showed similar responses to low P and eCO₂ interaction. This meta-analysis highlights crucial data gaps in capturing plant responses to eCO₂ and low P availability. Field-based experiments with longer-term exposure of both CO₂ and P manipulations are critically needed to provide ecosystem-scale understanding. Taken together, our results provide a quantitative baseline to constrain model-based hypotheses of plant responses to eCO₂ under P limitation, thereby improving projections of future global change impacts.

Keywords: biomass; carbon dioxide; leaf gas exchange; meta-analysis; mycorrhizae; nutrient concentration; plant morphology; plant nutrient uptake; soil phosphorus.