Effects of Glycosaminoglycan from Urechis unicinctus on ADP-Induced Platelet Calcium and Membrane Glycoprotein Expressions in Rats

Acta Haematol. 2021;144(1):44-47. doi: 10.1159/000505769. Epub 2020 Jul 10.

Abstract

Background: Previous studies had shown that glycosaminoglycan (GAG) from Urechis unicinctus exerts an obvious antiplatelet aggregation effect.

Objective: This study aims to investigate the effects of GAG from Urechis unicinctus on ADP-induced platelet calcium and membrane glycoprotein expressions in rats.

Methods: Fura-2/AM fluorescence probe was used to measure intracytosolic free-calcium concentration ([Ca2+]i) of platelets and calculate platelet calcium influx and release concentrations. Flow cytometry was used to detect the expressions of platelet membrane glycoproteins GPIIb/IIIa (PAC-1) and P-selectin (CD62P) in rats.

Results: The results showed that the GAG from U. unicinctus significantly inhibited the release of platelet calcium (p < 0.01) and the expressions of platelet GPII b/IIIa and P-selectin (p < 0.01) induced by ADP in rats but had no significant effect on the influx of platelet calcium (p > 0.01).

Conclusions: This study indicated that GAG may inhibit platelet activation and aggregation by reducing the release of Ca2+ and ADP-induced expression of platelet membrane glycoprotein in rats.

Keywords: GPIIb/IIIa; Glycosaminoglycan; P-selectin; Platelet calcium ion influx and release; Urechis unicinctus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Diphosphate / pharmacology*
  • Animals
  • Biological Products / chemistry
  • Biological Products / pharmacology*
  • Blood Platelets / drug effects*
  • Blood Platelets / metabolism*
  • Calcium / metabolism*
  • Calcium Signaling
  • Flow Cytometry
  • Gene Expression Regulation / drug effects*
  • Glycosaminoglycans / chemistry
  • Glycosaminoglycans / pharmacology*
  • Ions / metabolism
  • Platelet Membrane Glycoproteins / genetics*
  • Platelet Membrane Glycoproteins / metabolism
  • Polychaeta / chemistry*
  • Rats

Substances

  • Biological Products
  • Glycosaminoglycans
  • Ions
  • Platelet Membrane Glycoproteins
  • Adenosine Diphosphate
  • Calcium