A dual-response ratiometric fluorescent probe for hypochlorite and hydrazine detection and its imaging in living cells

Lin Wang; Qi Pan; Yuan Chen; Yunfu Ou; Huanyong Li; Bowen Li

doi:10.1016/j.saa.2020.118672

A dual-response ratiometric fluorescent probe for hypochlorite and hydrazine detection and its imaging in living cells

Spectrochim Acta A Mol Biomol Spectrosc. 2020 Nov 5:241:118672. doi: 10.1016/j.saa.2020.118672. Epub 2020 Jul 3.

Authors

Lin Wang¹, Qi Pan², Yuan Chen², Yunfu Ou¹, Huanyong Li³, Bowen Li⁴

Affiliations

¹ Analytical and Testing Center, Jinan University, Guangzhou 510632, PR China.
² College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, PR China.
³ Analytical and Testing Center, Jinan University, Guangzhou 510632, PR China. Electronic address: tlihuanyong@jnu.edu.cn.
⁴ College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, PR China. Electronic address: bowen_li@zju.edu.cn.

PMID: 32653825
DOI: 10.1016/j.saa.2020.118672

Abstract

In this work, a dual-response ratiometric fluorescent probe (E)-3-(5-(2-nitrovinyl)thiophen-2-yl)-9-phenyl-9H-carbazole (NTPC) for high selectivity and sensitivity detection of ClO^- and N₂H₄ was successfully developed. This probe NTPC showed ratiometric fluorescent response to ClO^- and N₂H₄, which induces obvious naked-eye color changes, respectively. In addition, the NTPC for ClO^- and N₂H₄ detection displayed low detection limits of 71.4 nM and 0.6 μM, respectively. And the sensing mechanism of NTPC with ClO^- and N₂H₄ was well confirmed by ¹H NMR and HR-MS spectra. Moreover, this novel probe was applied to monitoring and differentiating ClO^- and N₂H₄ in living cells, and exhibits good biocompatibility and low cytotoxicity.

Keywords: Carbazole; Cell imaging; Hydrazine detection; Hypochlorite detection; Ratiometric fluorescent.

MeSH terms

Diagnostic Imaging
Fluorescent Dyes*
HeLa Cells
Humans
Hydrazines / toxicity
Hypochlorous Acid*

Substances

Fluorescent Dyes
Hydrazines
Hypochlorous Acid