Therapeutic effects of conditioned - DPBS from amniotic stem cells on lactating cow mastitis

Wei-Je Ting; Steven W Shaw; Ling-Yien Hii; Tzu-Yi Lin; Shen-Chang Chang; Kang-You Liu; Perng-Chih Shen; Tzu-Jou Chen; Shao-Yu Peng

doi:10.1016/j.tjog.2020.05.009

Therapeutic effects of conditioned - DPBS from amniotic stem cells on lactating cow mastitis

Taiwan J Obstet Gynecol. 2020 Jul;59(4):520-526. doi: 10.1016/j.tjog.2020.05.009.

Authors

Wei-Je Ting¹, Steven W Shaw², Ling-Yien Hii³, Tzu-Yi Lin⁴, Shen-Chang Chang⁵, Kang-You Liu¹, Perng-Chih Shen¹, Tzu-Jou Chen¹, Shao-Yu Peng⁶

Affiliations

¹ Department of Animal Science, National Pingtung University of Science and Technology, Pingtung, Taiwan.
² College of Medicine, Chang Gung University, Taoyuan, Taiwan; Department of Obstetrics and Gynecology, Taipei Chang Gung Memorial Hospital, Taipei, Taiwan; Prenatal Cell and Gene Therapy Group, Institute for Women's Health, University College London, London, UK.
³ Department of Obstetrics and Gynecology, Sabah Women's and Children's Hospital, Sabah, Malaysia.
⁴ College of Medicine, Chang Gung University, Taoyuan, Taiwan.
⁵ Kaohsiung Animal Propagation Station, Livestock Research Institute, Council of Agriculture, Executive Yuan, Pingtung, Taiwan.
⁶ Department of Animal Science, National Pingtung University of Science and Technology, Pingtung, Taiwan. Electronic address: sypeng@mail.npust.edu.tw.

PMID: 32653123
DOI: 10.1016/j.tjog.2020.05.009

Abstract

Objective: Bovine mastitis results in economic loss due to decrease in milk production. Antibiotic ointments are commonly used for treating. However, residue and anti-microbial resistance warranted attention progressively. Fortunately, stem cell anti-inflammatory properties and paracrine expression of cytokines accelerates wound healing and suppresses inflammatory reactions in mastitis. The objective of this study is to use the conditioned-Dulbecco's pluripotent stem cells (DPBS) from amniotic membrane stem cells (AMSCs) in treating bovine mastitis.

Materials and methods: The cows with mastitis were divided into two groups. In antibiotic control group, the cows were given tetraneomycin ointment. In conditioned-DPBS of AMSCs treatment group, amniotic membrane was collected for AMSCs after delivery. With expression of surface antigen and potential of tri-linage differentiation, AMSCs were injected into mammary glands. Then, milk was sampled every three days to monitor the effect of both treatments. The quality of milk was measured with pH, titratable acidity, free calcium ions and somatic cell count.

Results: Our results demonstrated the Bovine AMSCs expressed CD44, low levels of CD4 and no CD105. Bovine AMSCs demonstrated the differentiation capability in the tri-cell lineages. Mastitis treatment with conditioned-DPBS from AMSCs (experimental group) and conventional antibiotics (control group) showed insignificant difference in pH value and titratable acidity. The level of ionic calcium concentration in the conditioned-DPBS group decreased from 3rd day to 12th day, while the level in the antibiotic group decreased from 0 day to 12th day. The somatic cell number was similar in both groups, which meet the standard of Taiwan milk collection.

Conclusion: In conclusion, conditioned-DPBS from bovine AMSCs has the therapeutic potential to treat bovine mastitis and may replace antibiotics therapy in the future.

Keywords: Amniotic membrane stem cells; Cytokines; Mastitis; Multipotent.

MeSH terms

Animals
Anti-Bacterial Agents / administration & dosage
Anti-Bacterial Agents / adverse effects
Cattle
Disease Models, Animal
Female
Humans
Lactation / immunology
Lactation / metabolism
Mammary Glands, Animal / metabolism
Mastitis, Bovine / therapy*
Milk / chemistry*
Ointments / administration & dosage
Pluripotent Stem Cells / transplantation*

Substances

Anti-Bacterial Agents
Ointments