Three new species of the Simulium (Simulium) striatum species-group (Diptera: Simuliidae) from Thailand, with their phylogenetic relationships

Kittipat Aupalee; Atiporn Saeung; Wichai Srisuka; Masako Fukuda; Anuluck Junkum; Benjawan Pitasawat; Hiroyuki Takaoka

doi:10.1016/j.actatropica.2020.105625

Three new species of the Simulium (Simulium) striatum species-group (Diptera: Simuliidae) from Thailand, with their phylogenetic relationships

Acta Trop. 2020 Nov:211:105625. doi: 10.1016/j.actatropica.2020.105625. Epub 2020 Jul 8.

Authors

Kittipat Aupalee¹, Atiporn Saeung², Wichai Srisuka³, Masako Fukuda⁴, Anuluck Junkum⁵, Benjawan Pitasawat⁶, Hiroyuki Takaoka⁷

Affiliations

¹ Graduate Doctoral Degree Program in Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. Electronic address: kittipat.aupalee@gmail.com.
² Center of Insect Vector Study, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: atiporn.s@cmu.ac.th.
³ Entomology Section, Queen Sirikit Botanic Garden, P.O. Box 7, Chiang Mai 50180, Thailand. Electronic address: wsrisuka@gmail.com.
⁴ Institute for Research Promotion, Oita University, Idaigaoka 1-1, Hasama, Yufu City, Oita, 879-5593, Japan. Electronic address: mfukuda@oita-u.ac.jp.
⁵ Center of Insect Vector Study, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: anuluck.j@cmu.ac.th.
⁶ Center of Insect Vector Study, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: benjawan.p@cmu.ac.th.
⁷ Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya, 50603, Kuala Lumpur, Malaysia. Electronic address: takaoka@oita-u.ac.jp.

PMID: 32649996
DOI: 10.1016/j.actatropica.2020.105625

Abstract

Three new species of black flies from Thailand, Simulium wangkwaiense, S. tadtonense and S. maeklongkeense, are described based on their adults, pupal exuviae and cocoons. All three new species are assigned to the Simulium (Simulium) striatum species-group, bringing its total number in Thailand to seven. Simulium wangkwaiense sp. nov. is the species formerly called S. quinquestriatum in Thailand. Certain male and pupal morphological characteristics are shown to separate all seven Thai species of this species-group. All of the three new species have been analyzed genetically for their phylogenetic relationships, with three known related species (except for S. thailandicum), by using the fast-evolving nuclear big zinc finger (BZF) gene.

Keywords: Aquatic insects; BZF; Simulium; Striatum species-group; Taxonomy.

MeSH terms

Animals
Female
Larva / anatomy & histology
Male
Phylogeny*
Pupa / anatomy & histology
Simuliidae / anatomy & histology
Simuliidae / classification*
Simuliidae / genetics
Species Specificity
Thailand