Metabolic signature of eyelid basal cell carcinoma

Jiancheng Huang; Jamie Schaefer; Yekai Wang; Lauren Gioia; Ying Pei; Xiaofei Shi; Shanawar Waris; Chen Zhao; John Nguyen; Jianhai Du

doi:10.1016/j.exer.2020.108140

Metabolic signature of eyelid basal cell carcinoma

Exp Eye Res. 2020 Sep:198:108140. doi: 10.1016/j.exer.2020.108140. Epub 2020 Jul 7.

Authors

Jiancheng Huang¹, Jamie Schaefer², Yekai Wang³, Lauren Gioia², Ying Pei⁴, Xiaofei Shi⁴, Shanawar Waris², Chen Zhao⁵, John Nguyen⁶, Jianhai Du⁷

Affiliations

¹ Eye Institute, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, 200031, China; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, and Shanghai Key Laboratory of Visual Impairment and Restoration (Fudan University), Shanghai, 200031, China; Department of Ophthalmology, West Virginia University, Morgantown, WV, 26506, USA; Department of Biochemistry, West Virginia University, Morgantown, WV, 26506, USA.
² Department of Ophthalmology, West Virginia University, Morgantown, WV, 26506, USA.
³ Department of Ophthalmology, West Virginia University, Morgantown, WV, 26506, USA; Department of Biochemistry, West Virginia University, Morgantown, WV, 26506, USA.
⁴ Department of Industrial and Management System Engineering, West Virginia University, Morgantown, WV, 26506, USA.
⁵ Eye Institute, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai, 200031, China; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, and Shanghai Key Laboratory of Visual Impairment and Restoration (Fudan University), Shanghai, 200031, China.
⁶ Department of Ophthalmology, West Virginia University, Morgantown, WV, 26506, USA; Department of Otolaryngology, West Virginia University, Morgantown, WV, 26506, USA. Electronic address: nguyenj@wvumedicine.org.
⁷ Department of Ophthalmology, West Virginia University, Morgantown, WV, 26506, USA; Department of Biochemistry, West Virginia University, Morgantown, WV, 26506, USA. Electronic address: jianhai.du@wvumedicine.org.

Abstract

Eyelid basal cell carcinoma (BCC) is the most common eyelid malignancy. Metabolic reprogramming is critical in tumorigenesis, but the metabolic feature of eyelid BCC remains elusive. In this study, we aim to reveal the metabolic profile in eyelid BCC using targeted metabolomics. Eyelid samples were collected from patients who had removal of BCC and from control patients who underwent blepharoplasty. Multivariate analysis of metabolomics data distinguished the two groups, indicating that eyelid BCC has significantly different metabolome than the healthy tissue. We found 16 increased and 11 decreased metabolites in the BCC tissues. These metabolites were highly enriched in the metabolism of nicotinamide adenine dinucleotide (NAD), glutathione metabolism, polyamine metabolism, and the metabolism of glycine, serine, threonine, arginine and proline. amino acid metabolism. Metabolites from NAD metabolism (Nicotinamide; Nicotinamide riboside; N1-Methylnicotinamide) had the highest sensitivity, specificity, and prediction accuracy in a prediction model for eyelid BCC. In conclusion, eyelid BCC has a signature change of cell metabolome. Metabolites in NAD metabolic pathways could potentially be biomarkers or therapeutic targets for eyelid BCC.

Keywords: Eyelid basal cell carcinoma; LC-MS/MS; Metabolism.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Aged
Biomarkers, Tumor / metabolism
Carcinoma, Basal Cell / metabolism*
Carcinoma, Basal Cell / pathology
Eyelid Neoplasms / metabolism*
Eyelid Neoplasms / pathology
Female
Humans
Male
Metabolome / physiology*
Metabolomics / methods*

Substances

Biomarkers, Tumor

Grants and funding

R01 EY026030/EY/NEI NIH HHS/United States