CD14/16 monocyte profiling in juvenile myelomonocytic leukemia

Pediatr Blood Cancer. 2020 Sep;67(9):e28555. doi: 10.1002/pbc.28555. Epub 2020 Jul 10.

Abstract

Monocyte subset analysis by flow cytometry has been shown to be a useful diagnostic tool in chronic myelomonocytic leukemia in adults. An increase in the classical monocyte fraction (CD14++/CD16-) greater than 94.0% of total monocytes is considered highly sensitive and specific in distinguishing chronic myelomonocytic leukemia from other myeloproliferative disorders. In a pilot study of juvenile myelomonocytic leukemia cases, we noted that CD14++/CD16- monocyte fraction was >95% in de novo juvenile myelomonocytic leukemia (JMML) with somatic PTPN11 mutations but normal in those with monosomy 7 or Noonan syndrome. Monocyte subgroup profiling by itself is not diagnostic of JMML but may distinguish molecular subgroups within JMML.

Keywords: flow cytometry; juvenile myelomonocytic leukemia; monocyte subsets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Child, Preschool
  • Female
  • Follow-Up Studies
  • GPI-Linked Proteins / metabolism
  • Humans
  • Infant
  • Leukemia, Myelomonocytic, Juvenile / metabolism*
  • Leukemia, Myelomonocytic, Juvenile / pathology*
  • Lipopolysaccharide Receptors / metabolism*
  • Male
  • Monocytes / metabolism*
  • Pilot Projects
  • Prognosis
  • Receptors, IgG / metabolism*
  • Retrospective Studies

Substances

  • CD14 protein, human
  • FCGR3B protein, human
  • GPI-Linked Proteins
  • Lipopolysaccharide Receptors
  • Receptors, IgG