NIR multiphoton ablation of cancer cells, fluorescence quenching and cellular uptake of dansyl-glutathione-coated gold nanoparticles

Sci Rep. 2020 Jul 9;10(1):11380. doi: 10.1038/s41598-020-68397-1.

Abstract

Theranostics based on two-photon excitation of therapeutics in the NIR region is an emerging and powerful tool in cancer therapy since this radiation deeply penetrates healthy biological tissues and produces selective cell death. Aggregates of gold nanoparticles coated with glutathione corona functionalized with the dansyl chromophore (a-DG-AuNPs) were synthesized and found efficient nanodevice for applications in photothermal therapy (PTT). Actually the nanoparticle aggregation enhances the quenching of radiative excitation and the consequent conversion into heat. The a-DG-AuNPs are readily internalized in Hep G2 where the chromophore acts as both antenna and transducer of the NIR radiation under two-photons excitation, determining efficient cell ablation via photothermal effect.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Combined Modality Therapy / instrumentation
  • Combined Modality Therapy / methods
  • Fluorescence
  • Glutathione / chemistry
  • Gold / chemistry
  • Humans
  • Infrared Rays / therapeutic use
  • Lasers
  • Low-Level Light Therapy / instrumentation
  • Low-Level Light Therapy / methods*
  • Metal Nanoparticles / administration & dosage*
  • Metal Nanoparticles / chemistry
  • Mice
  • Neoplasms / pathology
  • Neoplasms / therapy*
  • Phosphatidylcholines / chemistry
  • Photons / therapeutic use
  • Photothermal Therapy / instrumentation
  • Photothermal Therapy / methods*
  • Theranostic Nanomedicine / instrumentation
  • Theranostic Nanomedicine / methods*

Substances

  • 1-myristoyl-2-(12-((5-dimethylamino-1-naphthalenesulfonyl)amino)dodecanoyl)-sn-glycero-3-phosphocholine
  • Phosphatidylcholines
  • Gold
  • Glutathione