Transcriptome Sequencing Reveals the Genome Sequence of Pea Early Browning Virus from a 29-Year-Old Faba Bean Sample

S Maina; L Zheng; S King; V L McQueen; S L Norton; B Rodoni

doi:10.1128/MRA.00673-20

Transcriptome Sequencing Reveals the Genome Sequence of Pea Early Browning Virus from a 29-Year-Old Faba Bean Sample

Microbiol Resour Announc. 2020 Jul 9;9(28):e00673-20. doi: 10.1128/MRA.00673-20.

Authors

S Maina¹, L Zheng², S King³, V L McQueen³, S L Norton³, B Rodoni^{2

4}

Affiliations

¹ Australian Grains Genebank, Agriculture Victoria, Horsham, Victoria, Australia solomon.maina@agriculture.vic.gov.au.
² Agriculture Victoria, AgriBio, Melbourne, Victoria, Australia.
³ Australian Grains Genebank, Agriculture Victoria, Horsham, Victoria, Australia.
⁴ School of Applied Systems Biology, La Trobe University, Bundoora, Victoria, Australia.

Abstract

Pea early browning virus (PEBV) is transmitted by soil-inhabiting trichodorid nematodes and via seeds. The transcriptome sequencing method, followed by de novo assembly, revealed the PEBV Libyan isolate LyV66-91 genome. Its RNA1 resembled that of UK isolate SP5 with 93.91% nucleotide identity, and its RNA2 had 63.32% nucleotide identity to that of Dutch isolate E116.