Sequencing and Annotation of Duggie and Hocus, Two Subcluster B1 Mycobacteriophages

Erin L Doyle; Alexis N Burke; Samuel J Coy; Haley R Miller; Lilly M Shatford-Adams; Reagan M Petersen; Kade B Wehrs; Dane M Bowder

doi:10.1128/MRA.00432-20

Sequencing and Annotation of Duggie and Hocus, Two Subcluster B1 Mycobacteriophages

Microbiol Resour Announc. 2020 Jul 9;9(28):e00432-20. doi: 10.1128/MRA.00432-20.

Authors

Erin L Doyle¹, Alexis N Burke¹, Samuel J Coy¹, Haley R Miller¹, Lilly M Shatford-Adams¹, Reagan M Petersen¹, Kade B Wehrs¹, Dane M Bowder²

Affiliations

¹ Department of Biology, Doane University, Crete, Nebraska, USA.
² Department of Biology, Doane University, Crete, Nebraska, USA dane.bowder@doane.edu.

Abstract

Two mycobacteriophage genomes were newly sequenced and annotated. Duggie and Hocus were discovered, enriched, and isolated from soil using Mycobacterium smegmatis mc²155. The bacteriophages are lytic Siphoviridae and belong to the B1 subcluster. The Hocus and Duggie genomes are highly similar to one another in both nucleotide sequence and gene content.