A photoelectrochemical biosensor for rapid and ultrasensitive norovirus detection

Jiubiao Guo; Dan Liu; Zhiqiang Yang; Wenchuan Weng; Edward Wai Chi Chan; Zhenling Zeng; Kwok-Yin Wong; Peng Lin; Sheng Chen

doi:10.1016/j.bioelechem.2020.107591

A photoelectrochemical biosensor for rapid and ultrasensitive norovirus detection

Bioelectrochemistry. 2020 Dec:136:107591. doi: 10.1016/j.bioelechem.2020.107591. Epub 2020 Jul 1.

Authors

Jiubiao Guo¹, Dan Liu², Zhiqiang Yang³, Wenchuan Weng⁴, Edward Wai Chi Chan³, Zhenling Zeng⁵, Kwok-Yin Wong³, Peng Lin⁶, Sheng Chen⁷

Affiliations

¹ Guangdong Key Laboratory of Regional Immunity and Diseases, Department of Pathogen Biology, Shenzhen University School of Medicine, Shenzhen, China; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
² Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
³ Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Centre, The Hong Kong PolyU Shenzhen Research Institute, Shenzhen, China.
⁴ Department of Supervision on Import & Export Food Safety, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou, Guangdong, China.
⁵ Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
⁶ Shenzhen Key Laboratory of Special Functional Materials & Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, China. Electronic address: lin.peng@szu.edu.cn.
⁷ Shenzhen Key Laboratory for Food Biological Safety Control, Food Safety and Technology Research Centre, The Hong Kong PolyU Shenzhen Research Institute, Shenzhen, China; Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong. Electronic address: shechen@cityu.edu.hk.

PMID: 32645567
DOI: 10.1016/j.bioelechem.2020.107591

Abstract

The highly contagious norovirus (NoV) is the most common causative agent of acute gastroenteritis, resulting in >200,000 deaths worldwide annually. A rapid and sensitive detection method is a prerequisite for effective prevention and timely identification of NoV contamination. In the present study, we developed a photoelectrochemical (PEC) biosensor coupled with a novel custom-made monoclonal antibody (mAb) for specific and sensitive NoV detection. Our system could detect levels of recombinant NoV capsid protein VP1 as low as 2 × 10^-10 g mL^-1 (4.9 pM) within 30 min in a concentration-dependent manner. More importantly, the biosensor was versatile in detecting virus isolated from real samples that were as low as 46 copies μL^-1. These findings indicate that this system has the potential to serve as a convenient point-of-care system for diagnosing NoV infection and detecting NoV-contaminated food samples.

Keywords: Monoclonal antibody; Norovirus; Photoelectrochemical biosensor; QD; VP1.

MeSH terms

Antibodies, Monoclonal / immunology
Biosensing Techniques / methods
Electrochemical Techniques / instrumentation*
Limit of Detection
Norovirus / immunology
Norovirus / isolation & purification*
Photochemical Processes*

Substances

Antibodies, Monoclonal