Sensitivity of yeast to lithium chloride connects the activity of YTA6 and YPR096C to translation of structured mRNAs

Maryam Hajikarimlou; Houman Moteshareie; Katayoun Omidi; Mohsen Hooshyar; Sarah Shaikho; Tom Kazmirchuk; Daniel Burnside; Sarah Takallou; Narges Zare; Sasi Kumar Jagadeesan; Nathalie Puchacz; Mohan Babu; Myron Smith; Martin Holcik; Bahram Samanfar; Ashkan Golshani

doi:10.1371/journal.pone.0235033

Sensitivity of yeast to lithium chloride connects the activity of YTA6 and YPR096C to translation of structured mRNAs

PLoS One. 2020 Jul 8;15(7):e0235033. doi: 10.1371/journal.pone.0235033. eCollection 2020.

Authors

Maryam Hajikarimlou¹, Houman Moteshareie¹, Katayoun Omidi¹, Mohsen Hooshyar¹, Sarah Shaikho², Tom Kazmirchuk¹, Daniel Burnside¹, Sarah Takallou¹, Narges Zare¹, Sasi Kumar Jagadeesan¹, Nathalie Puchacz¹, Mohan Babu³, Myron Smith¹, Martin Holcik⁴, Bahram Samanfar^{1

5}, Ashkan Golshani¹

Affiliations

¹ Department of Biology and Ottawa Institute of Systems Biology, Carleton University, Ottawa, Ontario, Canada.
² Molecular Biomedicine Program, Children's Hospital of Eastern Ontario Research Institute, Ottawa, Ontario, Canada.
³ Department of Biochemistry, Research and Innovation Centre, University of Regina, Regina, Canada.
⁴ Department of Health Sciences, Carleton University, Ottawa, Ontario, Canada.
⁵ Agriculture and Agri-Food Canada, Ottawa Research and Development Centre (ORDC), Ottawa, Ontario, Canada.

Abstract

Lithium Chloride (LiCl) toxicity, mode of action and cellular responses have been the subject of active investigations over the past decades. In yeast, LiCl treatment is reported to reduce the activity and alters the expression of PGM2, a gene that encodes a phosphoglucomutase involved in sugar metabolism. Reduced activity of phosphoglucomutase in the presence of galactose causes an accumulation of intermediate metabolites of galactose metabolism leading to a number of phenotypes including growth defect. In the current study, we identify two understudied yeast genes, YTA6 and YPR096C that when deleted, cell sensitivity to LiCl is increased when galactose is used as a carbon source. The 5'-UTR of PGM2 mRNA is structured. Using this region, we show that YTA6 and YPR096C influence the translation of PGM2 mRNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphatases / genetics*
Adenosine Triphosphatases / metabolism
Antimanic Agents / pharmacology*
Gene Expression Regulation, Fungal / drug effects
Intracellular Signaling Peptides and Proteins / genetics
Intracellular Signaling Peptides and Proteins / metabolism*
Lithium Chloride / pharmacology*
Phosphoglucomutase / genetics
Protein Biosynthesis / drug effects
RNA, Messenger / genetics*
Saccharomyces cerevisiae / drug effects*
Saccharomyces cerevisiae / enzymology
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism*

Substances

Antimanic Agents
Intracellular Signaling Peptides and Proteins
RNA, Messenger
Saccharomyces cerevisiae Proteins
YPR096C protein, S cerevisiae
Adenosine Triphosphatases
YTA6 protein, S cerevisiae
Phosphoglucomutase
Lithium Chloride

Grants and funding

This research was funded by Natural Sciences and Engineering Research Council of Canada, NSERC grant number: 123456.