An Electrochemical Immunosensor for Sensitive Detection of the Tumor Marker Carcinoembryonic Antigen (CEA) Based on Three-Dimensional Porous Nanoplatinum/Graphene

Aihua Jing; Qiong Xu; Wenpo Feng; Gaofeng Liang

doi:10.3390/mi11070660

An Electrochemical Immunosensor for Sensitive Detection of the Tumor Marker Carcinoembryonic Antigen (CEA) Based on Three-Dimensional Porous Nanoplatinum/Graphene

Micromachines (Basel). 2020 Jul 3;11(7):660. doi: 10.3390/mi11070660.

Authors

Aihua Jing¹, Qiong Xu¹, Wenpo Feng¹, Gaofeng Liang²

Affiliations

¹ School of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang 471023, China.
² Medical College, Henan University of Science and Technology, Luoyang 471023, China.

Abstract

Carcinoembryonic antigen (CEA) is an important broad-spectrum tumor marker. The quantitative detection of a low concentration of CEA has important medical significance. In this study, three-dimensional porous graphene-oxide-supported platinum metal nanoparticles (3DPt/HGO) composites were prepared by a wet chemical method and modified on an electrode with enhanced conductivity, a large surface area, and good adsorption of immobilizing antibodies (Ab₁). Horseradish peroxidase (HRP)-functionalized Au nanoparticles were fabricated to label the secondary antibodies (Ab₂). The proposed immunosensor showed a good linear relationship in the range of 0.001-150 ng/mL for CEA and a detection limit of 0.0006 ng/mL. The immunosensor had high sensitivity, good stability and reproducibility, and has great application prospects for the clinical diagnosis of cancer.

Keywords: carcinoembryonic antigen (CEA); detection; electrochemical immunosensor; platinum (Pt); three-dimensional porous graphene; tumor marker.