Immunological and anticancer activities of seleno-ovalbumin (Se-OVA) on H22-bearing mice

Naxin Sun; Anguo Teng; Yana Zhao; Huiping Liu; Jianqiu Tu; Qi Jia; Qing Wang

doi:10.1016/j.ijbiomac.2020.07.006

Immunological and anticancer activities of seleno-ovalbumin (Se-OVA) on H22-bearing mice

Int J Biol Macromol. 2020 Nov 15:163:657-665. doi: 10.1016/j.ijbiomac.2020.07.006. Epub 2020 Jul 4.

Authors

Naxin Sun¹, Anguo Teng², Yana Zhao², Huiping Liu³, Jianqiu Tu², Qi Jia², Qing Wang²

Affiliations

¹ State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, PR China; Chinese Academy of Sciences, Tianjin Institute of Industrial Biotechnology, Tianjin 300308, PR China.
² State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, PR China.
³ State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, PR China. Electronic address: liuhuiping111@163.com.

PMID: 32634513
DOI: 10.1016/j.ijbiomac.2020.07.006

Abstract

Ovalbumin is the main protein component of egg white. Selenium is one of the essential trace elements. In our research, ovalbumin was modified into seleno-ovalbumin. After seleno-modification, the FTIR spectrum of seleno-ovalbumin appeared two new absorption peaks which belonged to the characteristic absorption peaks of Se-O and SeO. Seleno-ovalbumin could reduce the damage of cancer to immune organs, improve the proliferation capacities of T and B lymphocytes, enhance the NK cells cytotoxicity and increase the phagocytic activity of peritoneal macrophages of H22-bearing mice. Besides, Se-OVA could block the cell cycle of solid tumors cells in G0/G1 phase and accelerate the apoptosis of solid tumors cells through mitochondrial pathway.

Keywords: Anticancer; Ovalbumin; Seleno-modification.

MeSH terms

Animals
Antineoplastic Agents / chemistry*
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Biomarkers
Cell Cycle / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Disease Models, Animal
Female
Immunohistochemistry
Immunologic Factors / chemistry*
Immunologic Factors / pharmacology*
Lymphocyte Activation / immunology
Lymphocytes / immunology
Macrophages / drug effects
Macrophages / immunology
Macrophages / metabolism
Macrophages, Peritoneal / drug effects
Macrophages, Peritoneal / immunology
Macrophages, Peritoneal / metabolism
Mice
Mitochondria / drug effects
Organoselenium Compounds / chemistry*
Organoselenium Compounds / pharmacology*
Ovalbumin / chemistry*
Phagocytosis / drug effects
Phagocytosis / immunology
Spectroscopy, Fourier Transform Infrared
Xenograft Model Antitumor Assays

Substances

Antineoplastic Agents
Biomarkers
Immunologic Factors
Organoselenium Compounds
Ovalbumin