Characterization of Large Deletion Mutants of Mycobacterium tuberculosis Selected for Isoniazid Resistance

Antimicrob Agents Chemother. 2020 Aug 20;64(9):e00792-20. doi: 10.1128/AAC.00792-20. Print 2020 Aug 20.

Abstract

Large genomic deletions (LGDs) (6 to 63 kbp) were observed in isoniazid-resistant Mycobacterium tuberculosis mutants derived from four M. tuberculosis strains. These LGDs had no growth defect in vitro but could be defective in intracellular growth and showed various sensitivities toward oxidative stress despite lacking katG The LGD regions comprise 74 genes, mostly of unknown function, that may be important for M. tuberculosis intracellular growth and protection against oxidative stress.

Keywords: arginine; deletion; isoniazid; resistance.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Antitubercular Agents / pharmacology
  • Bacterial Proteins / genetics
  • Catalase / genetics
  • Drug Resistance, Bacterial / genetics
  • Isoniazid* / pharmacology
  • Mutation
  • Mycobacterium tuberculosis* / genetics

Substances

  • Antitubercular Agents
  • Bacterial Proteins
  • Catalase
  • Isoniazid