Direct monitoring of live human pluripotent stem cells by a highly selective pluripotency sensor

Myung Rae Park; Donggyu Nam; Hyunah Lee; Young-Tae Chang; Holm Zaehres; Jeong Beom Kim

doi:10.1016/j.bmcl.2020.127347

Direct monitoring of live human pluripotent stem cells by a highly selective pluripotency sensor

Bioorg Med Chem Lett. 2020 Aug 15;30(16):127347. doi: 10.1016/j.bmcl.2020.127347. Epub 2020 Jun 12.

Authors

Myung Rae Park¹, Donggyu Nam¹, Hyunah Lee¹, Young-Tae Chang², Holm Zaehres³, Jeong Beom Kim⁴

Affiliations

¹ School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, South Korea.
² Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang 37673, South Korea; Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, South Korea.
³ Department of Anatomy and Molecular Embryology, Ruhr-University Bochum, Bochum 44801, Germany.
⁴ School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, South Korea. Electronic address: jbkim@unist.ac.kr.

PMID: 32631546
DOI: 10.1016/j.bmcl.2020.127347

Abstract

Human pluripotent stem cells (hPSCs) are a useful cell source for regenerative medicine. Despite having a potential of hPSCs for cell-based therapy, there is a need for a selective human pluripotency sensor for monitoring of live hPSCs. Here, we report the discovery of a novel pluripotency sensor (SHI5) from BODIPY-based library by high-throughput cell-based screening and describe the use of SHI5 to identify and isolate human embryonic stem cells and human induced pluripotent stem cells. We demonstrate that SHI5-based assay can be applied to live cells that gain pluripotency in the reprogramming process without any effect on their viability. We also show that SHI5 is internalized through a clathrin-mediated endocytosis pathway. These findings suggest that SHI5 can be an attractive sensor for pluripotency cells during reprogramming. Taken together, SHI5-based screening for hPSCs opens probably unlimited possibilities of detection probe for hPSC therapy via assures their safety issue.

Keywords: BODIPY; DOFLA; Human pluripotent stem cells; Pluripotency; Sensor.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Fluorescent Dyes / chemistry*
High-Throughput Screening Assays*
Humans
Induced Pluripotent Stem Cells / cytology*
Molecular Structure

Substances

Fluorescent Dyes