Bioactive triterpenoids from Lantana camara showing anti-inflammatory activities in vitro and in vivo

Peng Wu; Ziteng Song; Xuelian Wang; Ying Li; Yuhao Li; Jianlin Cui; Muhetaer Tuerhong; Da-Qing Jin; Munira Abudukeremu; Dongho Lee; Jing Xu; Yuanqiang Guo

doi:10.1016/j.bioorg.2020.104004

Bioactive triterpenoids from Lantana camara showing anti-inflammatory activities in vitro and in vivo

Bioorg Chem. 2020 Aug:101:104004. doi: 10.1016/j.bioorg.2020.104004. Epub 2020 Jun 10.

Authors

Peng Wu¹, Ziteng Song¹, Xuelian Wang¹, Ying Li¹, Yuhao Li², Jianlin Cui², Muhetaer Tuerhong³, Da-Qing Jin², Munira Abudukeremu³, Dongho Lee⁴, Jing Xu⁵, Yuanqiang Guo⁶

Affiliations

¹ State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, and Drug Discovery Center for Infectious Disease, Nankai University, Tianjin 300050, People's Republic of China.
² School of Medicine, Nankai University, Tianjin 300071, People's Republic of China.
³ College of Chemistry and Environmental Sciences, Laboratory of Xinjiang Native Medicinal and Edible Plant Resources Chemistry, Kashgar University, Kashgar 844000, People's Republic of China.
⁴ Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.
⁵ State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, and Drug Discovery Center for Infectious Disease, Nankai University, Tianjin 300050, People's Republic of China. Electronic address: xujing611@nankai.edu.cn.
⁶ State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, and Drug Discovery Center for Infectious Disease, Nankai University, Tianjin 300050, People's Republic of China; State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, People's Republic of China. Electronic address: victgyq@nankai.edu.cn.

PMID: 32629274
DOI: 10.1016/j.bioorg.2020.104004

Abstract

Bioactive natural products play an important role in the research and development of new drugs. In our search for bioactive natural substances as potential lead compounds for inflammation, four new (1-4) and six known (6-10) triterpenoids were acquired from Lantana camara. Using NMR and MS techniques and electronic circular dichroism (ECD) calculations, these isolates were characterized and the new compounds (1-4) were found to be euphane-type triterpenoids. The in vitro anti-inflammatory effects of all the isolates were evaluated and the more bioactive compounds were selected for the investigation of preliminary mechanism using molecular docking and Western blotting experiments, as well as the in vivo anti-inflammatory evaluation using a zebrafish model.

Keywords: Anti-inflammatory properties; Euphane triterpenoids; Lantana camara; Molecular docking; Zebrafish model.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Inflammatory Agents / chemistry
Anti-Inflammatory Agents / pharmacology*
Blotting, Western
Cell Line
In Vitro Techniques
Lantana / chemistry*
Mice
Microglia / drug effects
Microglia / metabolism
Molecular Docking Simulation
Nitric Oxide / metabolism
Triterpenes / chemistry
Triterpenes / pharmacology*
Zebrafish

Substances

Anti-Inflammatory Agents
Triterpenes
Nitric Oxide