Two filamentous cyanobacteria of the genera Scytonema and Tolypothrix were reported to be effective for stabilizing soil in arid areas due to the production of significant amounts of extracellular polysaccharides (EPS). These EPS may also have applications in the biotechnology industry. Therefore, two cyanobacterial species, Scytonema tolypothrichoides and Tolypothrix bouteillei were examined using crossed gradients of temperature (8-40°C) and irradiance (3-21 W m-2) to identify their temperature and irradiance optima for maximum biomass and EPS production. According to their reported temperature requirements, both strains were considered mesophilic. The optimum growth range of temperature in S. tolypothrichoides (27 to 34°C) was higher than T. bouteillei (22-32°C). The optimum irradiance range for growth of S. tolypothrichoides (9-13 W m-2) was slightly lower than T. bouteillei (7-18 W m-2). Maximum EPS production by S. tolypothrichoides occurred at similar temperatures (28-34°C) as T. bouteillei (27-34°C), both slightly higher than for maximum growth. The optimum irradiance range for EPS production was comparable to that for growth in S. tolypotrichoides (8-13 W m-2), and slightly lower in T. bouteillei (7-17 W m-2). The Redundancy Analysis confirmed that temperature was the most important controlling factor and protocols for field applications or for mass cultivation can now be developed.
Keywords: biological soil crusts; cyanobacteria; extracellular polysaccharides (EPS); irradiance; temperature.
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