Immobilization of laccase onto modified PU/RC nanofiber via atom transfer radical polymerization method and application in removal of bisphenol A

Xin Li; Dawei Li; Pengfei Lv; Jinyan Hu; Quan Feng; Qufu Wei

doi:10.1002/elsc.201900075

Immobilization of laccase onto modified PU/RC nanofiber via atom transfer radical polymerization method and application in removal of bisphenol A

Eng Life Sci. 2019 Nov 4;19(11):815-824. doi: 10.1002/elsc.201900075. eCollection 2019 Nov.

Authors

Xin Li¹, Dawei Li¹, Pengfei Lv¹, Jinyan Hu², Quan Feng², Qufu Wei^{1

3}

Affiliations

¹ Key Laboratory of Eco-Textiles, Ministry of Education Jiangnan University Jiangsu Province Wuxi P. R. China.
² Key Laboratory of Textile Fabric Anhui Polytechnic University Wuhu Anhui P. R. China.
³ Fujian Key Laboratory of Novel Functional Textile Fiber and Materials Minjiang University Fuzhou Fujian P. R. China.

Abstract

In this study, 2-hydroxyethyl methacrylate (HEMA) was used as the monomers for surface grafting on electrospun PU/RC nanofiber membrane via atom transfer radical polymerization (ATRP) method, and the PU/RC-poly(HEMA) nanofiber membrane was investigated as a carrier for LAC. Free and immobilized LAC was characterized, and efficiency of bisphenol A (BPA) removal was determined. The results indicated that the PU/RC-poly(HEMA)-LAC showed relatively higher pH stability, temperature stability, and storage stability than free and PU/RC-LAC; moreover, more than 60% of the PU/RC-poly(HEMA)-LAC activity was retained after 10 cycles of ABTS treatment. Notably, the BPA removal efficiency of PU/RC-poly(HEMA)-LAC membrane generally ranged from 87.3 to 75.4% for the five cycles. Therefore, the PU/RC-poly(HEMA) nanofiber membrane has great potential as a carrier for the LAC immobilization for various industrial applications and bioremediation.

Keywords: ATRP; Laccase immobilization; bisphenol A; nanofiber.