Continuous enzymatic production and adsorption of laminaribiose in packed bed reactors

Akram Abi; Dave Hartig; Karl Vorländer; Anqi Wang; Stephan Scholl; Hans-Joachim Jördening

doi:10.1002/elsc.201800110

Continuous enzymatic production and adsorption of laminaribiose in packed bed reactors

Eng Life Sci. 2018 Oct 4;19(1):4-12. doi: 10.1002/elsc.201800110. eCollection 2019 Jan.

Authors

Akram Abi¹, Dave Hartig², Karl Vorländer², Anqi Wang¹, Stephan Scholl², Hans-Joachim Jördening¹

Affiliations

¹ Institute for Technical Chemistry Technische Universität Braunschweig Braunschweig Germany.
² Institute for Chemical and Thermal Process Engineering Technische Universität Braunschweig Braunschweig Germany.

Abstract

Bienzymatic production of laminaribiose from sucrose and glucose was combined with adsorption on zeolite BEA to introduce a first capture and purification step. Downstream processing including washing and desorption steps was characterized and optimized on a milliliter scale in batch mode. Results were then transferred to a packed bed system for enzymatic production and adsorption where the influence of adsorbent particle diameter on purity and productivity was evaluated. Finally, a continuous enzymatic production of laminaribiose was conducted over 10 days. The subsequent downstream processing of the loaded zeolites led to purities of over 0.5 g_{Laminaribiose} g_sugar ^-1 in the desorbate with a total productivity of 5.6 mg_{Laminaribiose} L_{enzyme bed} ^-1 h^-1 without the use of recycles.

Keywords: Adsorption; Continuous packed bed reactor; Downstream processing; Laminaribiose; Zeolite BEA.