Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration

Andreas Tosstorff; Cora Kroner; Diederik J Opperman; Frank Hollmann; Dirk Holtmann

doi:10.1002/elsc.201600158

Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration

Eng Life Sci. 2016 Oct 12;17(1):71-76. doi: 10.1002/elsc.201600158. eCollection 2017 Jan.

Authors

Andreas Tosstorff¹, Cora Kroner¹, Diederik J Opperman², Frank Hollmann³, Dirk Holtmann¹

Affiliations

¹ DECHEMA Research Institute Frankfurt Germany.
² Department of Biotechnology, University of the Free State Bloemfontein South Africa.
³ Department of Biotechnology TU Delft Julianalaan BL Delft The Netherlands.

Abstract

Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were identified.

Keywords: C=C reduction; Design of experiments; Electroenzymatic synthesis; Mediated electron transfer; Old yellow enzyme.