Human recombinant interleukin-1 regulates cellular mRNA levels of dermatan sulphate proteoglycan core protein

Biochem J. 1988 May 15;252(1):309-12. doi: 10.1042/bj2520309.

Abstract

Human skin fibroblasts were exposed to various concentrations (from 0.01 to 5.0 units/ml) of human recombinant interleukin-1 alpha and interleukin-1 beta (IL-1 alpha and IL-1 beta). Both IL-1 alpha and IL-1 beta were found to increase dermatan-sulphate-proteoglycan (DSPG) core-protein mRNA levels. Maximal increase (3.0-fold) was seen at 48 h after addition of 1 unit of IL-1 beta/ml. In spite of the elevated DSPG-core-protein mRNA only a slight increase (from 10 to 18%) could be seen in the production of DSPG to cell-culture medium. No changes in the molecular mass of DSPG could be detected.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aggrecans
  • Cells, Cultured
  • Electrophoresis, Agar Gel
  • Electrophoresis, Polyacrylamide Gel
  • Extracellular Matrix Proteins*
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Glycoproteins / biosynthesis*
  • Interleukin-1 / pharmacology*
  • Lectins, C-Type
  • Proteoglycans*
  • RNA, Messenger / metabolism*
  • Recombinant Proteins / pharmacology*
  • Skin / drug effects
  • Skin / metabolism

Substances

  • Aggrecans
  • Extracellular Matrix Proteins
  • Glycoproteins
  • Interleukin-1
  • Lectins, C-Type
  • Proteoglycans
  • RNA, Messenger
  • Recombinant Proteins