Sensitive detection of iron (II) sulfate with a novel reagent using spectrophotometry

Funda Ozkok; Yesim Muge Sahin; Vildan Enisoglu Atalay; Kamala Asgarova; Nihal Onul; Tunc Catal

doi:10.1016/j.saa.2020.118631

Sensitive detection of iron (II) sulfate with a novel reagent using spectrophotometry

Spectrochim Acta A Mol Biomol Spectrosc. 2020 Oct 15:240:118631. doi: 10.1016/j.saa.2020.118631. Epub 2020 Jun 20.

Authors

Funda Ozkok¹, Yesim Muge Sahin², Vildan Enisoglu Atalay³, Kamala Asgarova⁴, Nihal Onul⁴, Tunc Catal⁵

Affiliations

¹ Department of Chemistry, Istanbul University-Cerrahpasa, Avcilar, Istanbul, Turkey. Electronic address: ozkok@istanbul.edu.tr.
² Department of Biomedical Engineering, Istanbul Arel University, Turkey; Polymer Technologies and Composite Aplication and Research Center (ArelPOTKAM), Istanbul Arel University Buyukcekmece, Istanbul, Turkey. Electronic address: ymugesahin@arel.edu.tr.
³ Istanbul Protein Research Application and Inovation Center (PROMER), Turkey; Department of Bioengineering, Uskudar University, 34662 Uskudar, Istanbul, Turkey.
⁴ Department of Chemistry, Istanbul University-Cerrahpasa, Avcilar, Istanbul, Turkey.
⁵ Istanbul Protein Research Application and Inovation Center (PROMER), Turkey; Department of Molecular Biology and Genetics, Uskudar University, 34662 Uskudar, Istanbul, Turkey. Electronic address: tunc.catal@uskudar.edu.tr.

PMID: 32619787
DOI: 10.1016/j.saa.2020.118631

Abstract

In this study, a novel reagent was developed for sensitive detection of iron (II) sulfate, spectrophotometrically. A novel thio-anthraquinone derivative, 1-(Dodecylthio)anthracene-9,10-dione (3), was synthesized from the chemical reaction of 1-Chloroanthraquinone (1) and 1-Dodecanethiol (2) by an original reaction method and was used in the preparation of the novel reagent called Catal's reagent. A synthesized thio-anthraquinone analogue (3) was purified by column chromatography, and its chemical structure was characterized by spectroscopic methods such as Fourier-transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry (MS), and ultraviolet (UV)-visible spectrophotometry. The chemical and molecular structure of the developed thio-antraquinone derivative (3) was illuminated using computational methods with the GaussView5 and Gaussian09 programs. Various solvents including ethanol, methanol, and acetonitrile were examined in the preparation of the reagent. A concentration range from 0.2 mg mL^-1 up to 10 mg mL^-1 of iron (II) sulfate heptahydrate solution in distilled water was prepared. The absorption spectra of Catal's reagent (0.816 mM) showed three peaks between 185 nm-700 nm of wavelength. However, after the reaction with H₂O₂ and the 30 mM trisodium citrate dihydrate mixture in the presence of an iron sulfate (II) solution, a single peak was observed, producing a stable and reddish/brownish homogenous solution (λ max = 304 nm). The following concentrations of iron (II) sulfate heptahydrate was examined using developed protocol and the reagent, and the concentrations were measured spectrophotometrically at 304 nm, 0.2-1 mg mL^-1. Absorbances of reaction mixtures of iron (II) sulfate remained stable up to 48 h. The results indicated that the novel Catal's reagent can be used for sensitive spectrophotometric detection of iron (II) sulfate in aqueous solutions.

Keywords: 1-(Dodecylthio)anthracene-9,10-dione; Catal's reagent; Iron (II) sulfate; Spectrophotometer; Thiols.