Capillary zone electrophoresis (CZE) has been rising in its importance in structural analysis of plant cell walls, characterization of enzymes that degrade polysaccharides, and profiling of oligosaccharides to characterize cell wall mutants. CZE with laser-induced fluorescence detection provides high separation efficiencies, high-speed analysis, with extremely small sample requirements. Here we describe the instrumentation we use and methods for attaching fluorescent labels to oligosaccharides so that they can be detected.
Keywords: Capillary zone electrophoresis (CZE); Laser induced fluorescence detection; Oligosaccharide; Profiling; Separation.