This work was aimed to assess the contribution of classical nitrogen removal pathways in two deep constructed wetlands CW1 and CW2 located at Jaipur, India. Nitrogen mass balance revealed that 44.87% and 43.77% losses of T-N in CW1 and CW2 were unaccounted for. To elucidate these significant losses, the study was extended to assess the occurrence and contribution of a novel pathway (ANAMMOX) in overall nitrogen removal. The ratio of NH4+-N (removed) & NO3--N (produced) in CW1 & CW2 indicated that ANAMMOX could be one of the key pathways for nitrogen removal in the CWs besides nitrification-denitrification in microbial films. The molecular analysis confirmed bands of ANAMMOX bacteria developed intrinsically. The study revealed that deep wetlands can offer a feasible option for the sustenance of ANAMMOX bacteria and may help develop design parameters for CWs for achieving higherT-N removal withsimilarsurface area as that of conventional wetlands.
Keywords: Anaerobic ammonium oxidation (ANAMMOX); Constructed wetland; Denitrification; Nitrification; Nitrogen mass balance.
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