Precise imaging of mitochondria in cancer cells by real-time monitoring of nitroreductase activity with a targetable and activatable fluorescent probe

Ning Zhu; Ge Xu; Rongchen Wang; Tianli Zhu; Jiahui Tan; Xianfeng Gu; Chunchang Zhao

doi:10.1039/d0cc00494d

Precise imaging of mitochondria in cancer cells by real-time monitoring of nitroreductase activity with a targetable and activatable fluorescent probe

Chem Commun (Camb). 2020 Jul 14;56(56):7761-7764. doi: 10.1039/d0cc00494d.

Authors

Ning Zhu¹, Ge Xu¹, Rongchen Wang¹, Tianli Zhu¹, Jiahui Tan², Xianfeng Gu², Chunchang Zhao¹

Affiliations

¹ Key Laboratory for Advanced Materials and Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China. zhaocchang@ecust.edu.cn.
² Department of Medicinal Chemistry, School of Pharmacy, Fudan University, Shanghai, 201203, P. R. China. xfgu@fudan.edu.cn.

PMID: 32613955
DOI: 10.1039/d0cc00494d

Abstract

An activatable and mitochondrial-targetable fluorescent probe was developed. This designed probe showed ratiometric fluorescence and light-up near-infrared emission responsiveness to nitroreductase, achieving precise imaging of mitochondria in cancer cells by real-time monitoring of nitroreductase activity.

MeSH terms

A549 Cells
Fluorescent Dyes / chemistry*
Humans
Mitochondria / metabolism*
Neoplasms / metabolism*
Nitroreductases / metabolism*
Optical Imaging / methods*

Substances

Fluorescent Dyes
Nitroreductases