Data on differentially expressed proteins in rock inhibitor-treated human trabecular meshwork cells using SWATH-based proteomics

Sze-Wan Shan; Chi-Wai Do; Thomas Chuen Lam; Hoi-Lam Li; W Daniel Stamer; Chi-Ho To

doi:10.1016/j.dib.2020.105846

Data on differentially expressed proteins in rock inhibitor-treated human trabecular meshwork cells using SWATH-based proteomics

Data Brief. 2020 Jun 12:31:105846. doi: 10.1016/j.dib.2020.105846. eCollection 2020 Aug.

Authors

Sze-Wan Shan¹, Chi-Wai Do¹, Thomas Chuen Lam^{1

2}, Hoi-Lam Li¹, W Daniel Stamer^{3

4}, Chi-Ho To¹

Affiliations

¹ Laboratory of Experimental Optometry, Centre for Myopia Research, School of Optometry, the Hong Kong Polytechnic University, Kowloon, Hong Kong, China.
² The Hong Kong Polytechnic University Shenzhen Research Institute, Shenzhen, China.
³ Department of Ophthalmology, Duke University, Durham, NC, United States.
⁴ Department of Biomedical Engineering, Duke University,Durham, NC, United States.

Abstract

Rho-associated coiled coil-forming protein kinase (ROCK) inhibitors represent a novel class of anti-glaucoma drugs because of their ocular hypotensive effects. However, the underlying mechanisms responsible for lowering intraocular pressure (IOP) are not completely clear. The protein profile changes in primary human trabecular meshwork (TM) cells after two days treatment with a ROCK inhibitor were studied using label-free SWATH acquisition. These results provided significant data of key protein candidates underlying the effect of ROCK inhibitor. Using the sensitive label-free mass spectrometry approach with data-independent acquisition (SWATH-MS), we established a comprehensive TM proteome library. All raw data generated from IDA and SWATH acquisitions were uploaded and published in the Peptide Atlas public repository (http://www.peptideatlas.org/) for general release (Data ID PASS01254).

Keywords: Rock inhibitor; Swath; Trabecular meshwork; glaucoma.