As an effective natural antibacterial component, the low water solubility of thymol (THY) has stemmed its potential in biomedical application. Here, β-cyclodextrin (β-CD) and THY were self-assembled to form water-soluble inclusion complex (IC). The successful formation of IC was confirmed via 1H NMR. As an antibacterial agent, the resultant IC was then incorporated into cellulose acetate (CA) fibrous matrix with hierarchical structure (nanopores on porous fibrous webs) via electrospinning (CA/THY/β-CD), and the pure THY was also encapsulated into CA for comparison (CA/THY). In vitro dissolution tests demonstrated that CA/THY/β-CD fibrous membrane exhibited sustained drug release, which abided by non-Fickian diffusion. Besides, the CA/THY/β-CD fibrous membrane exhibited more effective and long-lasting antibacterial activity against S. aureus. Furthermore, the combination of hierarchical porous structure with sustained drug release endowed the CA/THY/β-CD fibrous membrane with good cytocompatibility. Taken together, the CA/THY/β-CD fibrous membrane could be an attractive candidate for wound dressing material.
Keywords: Antibacterial activity; Drug release; Hierarchical porous structure; Thymol; β-Cyclodextrin.
Copyright © 2020. Published by Elsevier B.V.