Mechanical Rupture-Based Antibacterial and Cell-Compatible ZnO/SiO2 Nanowire Structures Formed by Bottom-Up Approaches

Taisuke Shimada; Takao Yasui; Akihiro Yonese; Takeshi Yanagida; Noritada Kaji; Masaki Kanai; Kazuki Nagashima; Tomoji Kawai; Yoshinobu Baba

doi:10.3390/mi11060610

Mechanical Rupture-Based Antibacterial and Cell-Compatible ZnO/SiO₂ Nanowire Structures Formed by Bottom-Up Approaches

Micromachines (Basel). 2020 Jun 24;11(6):610. doi: 10.3390/mi11060610.

Authors

Taisuke Shimada¹, Takao Yasui^{1

2

3}, Akihiro Yonese¹, Takeshi Yanagida^{4

5

6}, Noritada Kaji^{2

7}, Masaki Kanai⁶, Kazuki Nagashima^{3

4}, Tomoji Kawai⁵, Yoshinobu Baba^{1

2

8}

Affiliations

¹ Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
² Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
³ Japan Science and Technology Agency (JST), PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
⁴ Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
⁵ The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan.
⁶ Institute for Materials Chemistry and Engineering, Kyushu University, 6-1 Kasuga-koen, Kasuga, Fukuoka 816-8580, Japan.
⁷ Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
⁸ Institute of Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology, Anagawa 4-9-1, Inage-ku, Chiba 263-8555, Japan.

Abstract

There are growing interests in mechanical rupture-based antibacterial surfaces with nanostructures that have little toxicity to cells around the surfaces; however, current surfaces are fabricated via top-down nanotechnologies, which presents difficulties to apply for bio-surfaces with hierarchal three-dimensional structures. Herein, we developed ZnO/SiO₂ nanowire structures by using bottom-up approaches and demonstrated to show mechanical rupture-based antibacterial activity and compatibility with human cells. When Escherichia coli were cultured on the surface for 24 h, over 99% of the bacteria were inactivated, while more than 80% of HeLa cells that were cultured on the surface for 24 h were still alive. This is the first demonstration of mechanical rupture-based bacterial rupture via the hydrothermally synthesized nanowire structures with antibacterial activity and cell compatibility.

Keywords: antibacterial activity; cell compatibility; hydrothermal synthesis; mechanical-based rupture; nanowire structures.

Abstract

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