Design and evaluation of the anticancer activity of paclitaxel-loaded anisotropic-poly(lactic-co-glycolic acid) nanoparticles with PEGylated chitosan surface modifications

Abstract

This study aimed to evaluate the anticancer activity of paclitaxel-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (PNPs) based on their shapes and surface modifications in breast cancer cells. We hypothesized that anisotropic-PNPs (AT-PNPs) with PEGylated chitosan (CP) surface modifications and high aspect ratios exhibit higher anticancer activity than PNPs and AT-PNPs with CP surface modifications and low aspect ratios. Six types of PNPs and AT-PNPs with different shapes and surface modifications were successfully prepared. The cellular uptake and cytotoxicity of the AT-PNPs were higher than those of the PNPs, while the cellular uptake and cytotoxicity of the PNPs and AT-PNPs with CP were higher than those of the uncoated PNPs and AT-PNPs. Moreover, all the particles remained stable for 4 months. In conclusion, this study primarily described the preparation of CP-AT-PNPs, and the CP-AT-PNPs2 developed herein are expected to demonstrate promising anticancer effects in animal experiments and clinical studies.

Keywords: Anisotropic-PLGA nanoparticles; Anticancer effects; PEGylated chitosan.