Regular exercise improves the health status of dogs; however, extreme exertion in the absence of adequate fluid and electrolyte replacement may negatively impact health and performance due to dehydration and cardiovascular stress. Unlike humans and horses, dogs thermoregulate predominantly through respiration and salivation, yet there is a dearth of literature defining exercise-induced changes to canine salivary electrolytes. The study objective was to investigate the effects of exercise on salivary electrolyte concentrations, and to determine if adaptations may occur in response to incremental conditioning in client-owned Siberian Huskies. Sixteen dogs were used, with an average age of 4.8 ± 2.5 years and body weight of 24.3 ± 4.3 kg. A 12-week exercise regimen was designed to increase in distance each week, but weather played a role in setting the daily distance. Saliva samples were collected at weeks 0 (pre-run, 5.7 km), 5 (pre-run, 5.7, 39.0 km), and 11 (pre-run, 5.7, 39.0 km). Samples were analyzed for sodium, chloride, potassium, calcium, magnesium, and phosphorous using photometric and indirect ion-selective electrode analysis. When compared across weeks, sodium, chloride, potassium, and calcium concentrations did not differ at any sampling time point; however, phosphorus and magnesium concentrations increased from baseline. Data were then pooled across weeks to evaluate changes due to distance and level of conditioning. Sodium, chloride, and magnesium concentrations increased progressively with distance ran, suggesting that these electrolytes are primarily being lost as exercising dogs salivate. Repletion of these minerals may assist in preventing exercise-induced electrolyte imbalance in physically active dogs.
Keywords: electrolyte minerals; exercise; saliva; sporting dogs.
© 2020 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.