Effect of Curcumol on the Fenestrae of Liver Sinusoidal Endothelial Cells Based on NF- κ B Signaling Pathway

Yang Zheng; Jiahui Wang; Jiaru Wang; Haiyuan Xie; Tiejian Zhao

doi:10.1155/2020/8590638

Effect of Curcumol on the Fenestrae of Liver Sinusoidal Endothelial Cells Based on NF- κ B Signaling Pathway

Evid Based Complement Alternat Med. 2020 May 28:2020:8590638. doi: 10.1155/2020/8590638. eCollection 2020.

Authors

Yang Zheng¹, Jiahui Wang¹, Jiaru Wang², Haiyuan Xie³, Tiejian Zhao³

Affiliations

¹ Department of Medicine, Faculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning, Guangxi 530021, China.
² College of Nursing, Guangdong Medical University, Dongguan, Guangdong 523000, China.
³ Department of Physiology, College of Basic Medicine, Guangxi University of Chinese Medicine, Nanning, Guangxi 530021, China.

Abstract

Objective: To study the effect of curcumol on liver sinusoidal endothelial cells (LSECs) and to analyze the mechanism of antihepatic fibrosis.

Methods: The effects of drug intervention on cell proliferation rates were detected by MTT assay. The expression of NF-κB was detected by RT-PCR and WB. The NF-κB expression and entry into the nucleus were detected by immunofluorescence; scanning electron microscopy was used to observe the changes of LSECs fenestrae.

Results: MTT results showed that the interference of cell proliferation in each group was small. RT-PCR showed that the expression of NF-κB in the curcumol intervention group was significantly lower than that in the positive control group (P < 0.05). The WB detection found that, in the curcumol intervention group, the expression of pNF-κB in the NF-κB signaling pathway was significantly lower than that in the positive control group (P < 0.05). Scanning electron microscopy showed that the LSEC fenestrae were significantly improved compared with the positive control group.

Conclusion: Curcumol may be one of the mechanisms of antihepatic fibrosis by inhibiting the activity of the NF-κB signaling pathway and increasing the fenestrae of LSECs.