A 3'-tRNA-derived fragment enhances cell proliferation, migration and invasion in gastric cancer by targeting FBXO47

Fei Zhang; Jinxin Shi; Zhonghua Wu; Peng Gao; Weixu Zhang; Bicheng Qu; Xin Wang; Yongxi Song; Zhenning Wang

doi:10.1016/j.abb.2020.108467

A 3'-tRNA-derived fragment enhances cell proliferation, migration and invasion in gastric cancer by targeting FBXO47

Arch Biochem Biophys. 2020 Sep 15:690:108467. doi: 10.1016/j.abb.2020.108467. Epub 2020 Jun 24.

Authors

Fei Zhang¹, Jinxin Shi¹, Zhonghua Wu¹, Peng Gao¹, Weixu Zhang¹, Bicheng Qu¹, Xin Wang¹, Yongxi Song², Zhenning Wang³

Affiliations

¹ Department of Surgical Oncology and General Surgery, Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang City, 110001, China.
² Department of Surgical Oncology and General Surgery, Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang City, 110001, China. Electronic address: songyongxi840309@126.com.
³ Department of Surgical Oncology and General Surgery, Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, The First Affiliated Hospital of China Medical University, 155 North Nanjing Street, Heping District, Shenyang City, 110001, China. Electronic address: josieon826@sina.cn.

PMID: 32592804
DOI: 10.1016/j.abb.2020.108467

Abstract

Increasing evidence demonstrates that tRNA-derived fragments (tRFs) exert important effects and are dysregulated in various human cancer types. However, their roles in gastric cancer (GC) remain unknown. Here we identified the functional effects of tRF-3019a (derived from tRNA-Ala-AGC-1-1) in GC. We demonstrated that tRF-3019a was upregulated in GC tissues and cell lines. Phenotypic studies revealed that tRF-3019a overexpression enhances GC cell proliferation, migration and invasion. Conversely, tRF-3019a knockdown inhibits GC cell malignant activities. Mechanistic investigation implies that tRF-3019a directly regulates tumor suppressor gene FBXO47. Furthermore, tRF-3019a levels may discriminate GC tissues from nontumorous tissues. Taken together, our results reveal that tRF-3019a modulates GC cell proliferation, migration and invasion by targeting FBXO47, and it may serve as a potential diagnostic biomarker for GC.

Keywords: Biomarker; FBXO47; Gastric cancer; tRNA-derived fragment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Biomarkers, Tumor / metabolism
Cell Line, Tumor
Cell Movement
Cell Proliferation
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
Humans
Nucleic Acid Conformation
RNA, Transfer / metabolism*
RNA, Transfer, Amino Acid-Specific / genetics*
Stomach Neoplasms / metabolism*
Transcription Factors / genetics*
Transcription Factors / metabolism*
Transfection

Substances

Biomarkers, Tumor
FBXO47 protein, human
RNA, Transfer, Amino Acid-Specific
Transcription Factors
RNA, Transfer