A fluorescent molecular rotor probe for tracking plasma membranes and exosomes in living cells

Ling Feng; Yusheng Xie; Sung King Au-Yeung; Hagos Birhanu Hailu; Zhiyang Liu; Qingxin Chen; Jie Zhang; Qiuxiang Pang; Xi Yao; Mengsu Yang; Liang Zhang; Hongyan Sun

doi:10.1039/d0cc03069d

A fluorescent molecular rotor probe for tracking plasma membranes and exosomes in living cells

Chem Commun (Camb). 2020 Jul 28;56(60):8480-8483. doi: 10.1039/d0cc03069d.

Authors

Affiliations

¹ Cancer and Aging Research Institution, School of Life Science, Shandong University of Technology, China and Department of Chemistry and COSDAF (Centre of Super-Diamond and Advanced Films), City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China. yushengxie@outlook.com hongysun@cityu.edu.hk and Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057, P. R. China.
² Department of Chemistry and COSDAF (Centre of Super-Diamond and Advanced Films), City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China. yushengxie@outlook.com hongysun@cityu.edu.hk and Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057, P. R. China.
³ Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057, P. R. China and Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China. liangzhang.28@cityu.edu.hk.
⁴ Cancer and Aging Research Institution, School of Life Science, Shandong University of Technology, China.

PMID: 32588854
DOI: 10.1039/d0cc03069d

Abstract

A rotor-based probe MRMP-1 was designed and synthesized. MRMP-1 can bind to plasma membranes very quickly and stably with remarkable fluorescence enhancement. It can be used to monitor the dynamic changes in cell membranes in real-time under stimuli conditions. Importantly, MRMP-1 is the first rotor-based fluorescent sensor to label exosomes in living cells.

MeSH terms

Animals
Cell Line
Cell Membrane / chemistry*
Cell Membrane / metabolism
Exosomes / chemistry*
Exosomes / metabolism
Fluorescent Dyes / chemistry*
Humans
Hydrogen Peroxide / chemistry
Mice
Microscopy, Confocal
Spectrometry, Fluorescence

Substances

Fluorescent Dyes
Hydrogen Peroxide