Trigonelline-loaded chitosan nanoparticles prompted antitumor activity on glioma cells and biocompatibility with pheochromocytoma cells

Anbazhagan Sathiyaseelan; Kandasamy Saravanakumar; Jothi Jayalakshmi; Margabandhu Gopi; Azeez Shajahan; Kaliyan Barathikannan; Pudupalayam Thangavelu Kalaichelvan; Myeong-Hyeon Wang

doi:10.1016/j.ijbiomac.2020.06.165

Trigonelline-loaded chitosan nanoparticles prompted antitumor activity on glioma cells and biocompatibility with pheochromocytoma cells

Int J Biol Macromol. 2020 Nov 15:163:36-43. doi: 10.1016/j.ijbiomac.2020.06.165. Epub 2020 Jun 22.

Authors

Anbazhagan Sathiyaseelan¹, Kandasamy Saravanakumar², Jothi Jayalakshmi³, Margabandhu Gopi⁴, Azeez Shajahan⁵, Kaliyan Barathikannan⁶, Pudupalayam Thangavelu Kalaichelvan⁵, Myeong-Hyeon Wang⁷

Affiliations

¹ Centre for Advanced Studies in Botany, University of Madras, Chennai 600025, Tamil Nadu, India; Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea. Electronic address: sathiyaseelan.bio@gmail.com.
² Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
³ Department of Biochemistry, University of Madras, Chennai 600025, Tamil Nadu, India.
⁴ National Brain Research Centre (NBRC), Gurgaon, Haryana 122051, India.
⁵ Centre for Advanced Studies in Botany, University of Madras, Chennai 600025, Tamil Nadu, India.
⁶ Research Department of Plant Biology and Biotechnology, Loyola College, Chennai 600034, Tamil Nadu, India.
⁷ Department of Medical Biotechnology, College of Biomedical Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea. Electronic address: mhwang@kangwon.ac.kr.

PMID: 32585274
DOI: 10.1016/j.ijbiomac.2020.06.165

Abstract

Trigonelline-loaded water-soluble chitosan nanoparticles (Trigo-WSCS NPs) were prepared for the treatment of glioblastoma (targeting C6 glioma cells) and also evaluated its biocompatibility with rat adrenal pheochromocytoma cells (PC12 cells). WSCS-Trigo NPs characteristics were determined using UV-Visible spectrophotometer, FTIR, XRD, TEM, DLS, and Zeta potential. Trigo-WSCS NPs were noted to have a spherical shape, with an average size of 356 nm. Trigo-WSCS NPs zeta potential was 30.9 mv, which expresses its good stability. The WSCS-Trigo NPs considerably inhibited the growth of rat C6 glioma cells and exhibited an IC₅₀ concentration of 34 μg/mL. Further, Trigo-WSCS NPs were biocompatible with PC12 cells in terms of enhancing neurite growth and differentiation. In conclusion, Trigo-WSCS NPs could act as an antitumor drug for the treatment of glioblastoma as suggested by the in vitro studies.

Keywords: Biocompatibility; Glioblastoma; Nanoparticles; TEM; Trigonelline; Water soluble chitosan.

MeSH terms

Adrenal Gland Neoplasms / metabolism
Alkaloids / administration & dosage
Alkaloids / chemistry*
Alkaloids / pharmacology*
Animals
Antineoplastic Agents / administration & dosage
Antineoplastic Agents / chemistry*
Antineoplastic Agents / pharmacology*
Apoptosis / drug effects
Cell Cycle / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Chitosan / administration & dosage
Chitosan / chemistry*
Drug Carriers / administration & dosage
Drug Carriers / chemical synthesis
Drug Carriers / chemistry*
Drug Liberation
Flow Cytometry
Glioblastoma / drug therapy
Glioblastoma / metabolism
Glioblastoma / pathology
Glioma / drug therapy
Glioma / metabolism
Glioma / pathology
Materials Testing
Microscopy, Fluorescence
Nanoparticles / administration & dosage
Nanoparticles / analysis
Nanoparticles / chemistry*
Particle Size
Pheochromocytoma / metabolism
Rats

Substances

Alkaloids
Antineoplastic Agents
Drug Carriers
trigonelline
Chitosan