Application of Various Delivery Methods for CRISPR/dCas9

Mol Biotechnol. 2020 Aug;62(8):355-363. doi: 10.1007/s12033-020-00258-8. Epub 2020 Jun 24.

Abstract

As gene-editing technology has become more and more popular in the life sciences, CRISPR has brought good news to scientific researchers because of its efficiency, convenience, and wide application. Its wide application has also promoted the development of basic scientific research, agriculture, basic medicine, and clinical treatment. However, how the CRISPR/dCas9 system is effectively delivered to the target organs or cells is still unknown. This paper briefly introduces the CRISPR/dCas9 system and then lists some common delivery methods and their characteristics.

Keywords: CRISPR; Delivery; dCas9.

Publication types

  • Review

MeSH terms

  • Animals
  • CRISPR-Cas Systems*
  • Drug Carriers
  • Drug Delivery Systems*
  • Electroporation
  • Gene Editing*
  • Genetic Vectors
  • Humans
  • Mice

Substances

  • Drug Carriers