Nitrification Inhibitor 3,4-Dimethylpyrazole Phosphate Application During the Later Stage of Apple Fruit Expansion Regulates Soil Mineral Nitrogen and Tree Carbon-Nitrogen Nutrition, and Improves Fruit Quality

Abstract

In order to solve the problems of nitrogen (N) losses and fruit quality degradation caused by excessive N fertilizer application, different dosages of the nitrification inhibitor, 3,4-dimethylpyrazole phosphate (DMPP) (0, 0.5, 1, 2, and 4 mg kg^-1 soil), were applied during the later stage of 'Red Fuji' apple (Malus domestica Borkh.) fruit expansion in 2017 and 2018. The effects of DMPP on soil N transformation, carbon (C)-N nutrition of tree, and fruit quality were investigated. Results revealed that DMPP decreased the abundance of ammonia-oxidizing bacteria (AOB) amoA gene, increased the retention of NH₄ ⁺-N, and decreased NO₃ ^--N concentration and its vertical migration in soil. DMPP reduced ¹⁵N loss rates and increased ¹⁵N residual and recovery rates compared to the control. ¹³C and ¹⁵N double isotope labeling results revealed that DMPP reduced the capacity of ¹⁵N absorption and regulation in fruits, decreased ¹⁵N accumulation in fruits and whole plant, and increased the distribution of ¹³C from vegetative organs to fruits. DMPP increased fruit anthocyanin and soluble sugar contents, and had no significant effect on fruit yield. The comprehensive analysis revealed that the application of 1 mg DMPP kg^-1 soil during the later stage of fruit expansion effectively reduced losses due to N and alleviated quality degradation caused by excessive N fertilizer application.

Keywords: 13C; 15N; DMPP; apple; fruit quality; mineral nitrogen.