Interleukin-8 Release Inhibitors Generated by Fermentation of Artemisia princeps Pampanini Herb Extract With Lactobacillus plantarum SN13T

Tomoko Okamoto; Sachiko Sugimoto; Masafumi Noda; Tomoharu Yokooji; Narandalai Danshiitsoodol; Fumiko Higashikawa; Masanori Sugiyama

doi:10.3389/fmicb.2020.01159

Interleukin-8 Release Inhibitors Generated by Fermentation of Artemisia princeps Pampanini Herb Extract With Lactobacillus plantarum SN13T

Front Microbiol. 2020 Jun 3:11:1159. doi: 10.3389/fmicb.2020.01159. eCollection 2020.

Authors

Tomoko Okamoto^{1

2}, Sachiko Sugimoto³, Masafumi Noda¹, Tomoharu Yokooji², Narandalai Danshiitsoodol¹, Fumiko Higashikawa¹, Masanori Sugiyama¹

Affiliations

¹ Department of Probiotic Science for Preventive Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
² Department of Frontier Science for Pharmacotherapy, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.
³ Department of Pharmacognosy, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan.

Abstract

Some glycosides, which are detected in water extracts from medicinal plants, have been reported to be degraded into their aglycones by incubating with some microorganisms producing β-glucosidase. We have shown that a plant-derived Lactobacillus plantarum SN13T harbors 11 open reading frames (ORFs) encoding the β-glucosidase enzyme and can grow vigorously in several herbal water extracts. In this study, we observed that the water extract from Artemisia princeps Pampanini (AP) fermented with the SN13T strain strongly inhibited the release of interleukin (IL)-8 from the HuH-7 cells, when compared to that without fermentation. Furthermore, we demonstrated that the SN13T strain produced at least two bioactive compounds from some compounds contained in AP extract. In addition, we determined that the two compounds were catechol and seco-tanapartholide C, which dose-dependently inhibited the release of IL-8. Because some sesquiterpene lactones are useful in pharmaceuticals, seco-tanapartholide C may be useful as an anti-inflammatory agent. This study suggests that the fermentation of medicinal herbs with Lb. plantarum SN13T is a significant technique to obtain bioactive compounds having therapeutic potential.

Keywords: Artemisia princeps Pampanini; Lactobacillus plantarum; lactic acid bacteria; medicinal plant; sesquiterpene lactone.