Sensing cellular biochemistry with fluorescent chemical-genetic hybrids

Arnaud Gautier; Alison G Tebo

doi:10.1016/j.cbpa.2020.04.005

Sensing cellular biochemistry with fluorescent chemical-genetic hybrids

Curr Opin Chem Biol. 2020 Aug:57:58-64. doi: 10.1016/j.cbpa.2020.04.005. Epub 2020 Jun 21.

Authors

Arnaud Gautier¹, Alison G Tebo²

Affiliations

¹ Sorbonne Université, École Normale Supérieure, PSL University, CNRS, Laboratoire des Biomolécules, LBM, 75005 Paris, France; Institut Universitaire de France, France. Electronic address: arnaud.gautier@sorbonne-universite.fr.
² Sorbonne Université, École Normale Supérieure, PSL University, CNRS, Laboratoire des Biomolécules, LBM, 75005 Paris, France. Electronic address: teboa@janelia.hhmi.org.

PMID: 32580134
DOI: 10.1016/j.cbpa.2020.04.005

Abstract

Fluorescent biosensors are powerful tools for the detection of biochemical events inside cells with high spatiotemporal resolution. Biosensors based on fluorescent proteins often suffer from issues with photostability and brightness. On the other hand, hybrid, chemical-genetic systems present unique opportunities to combine the strengths of synthetic, organic chemistry with biological macromolecules to generate exquisitely tailored semisynthetic sensors.

Keywords: Biosensing; Fluorescence imaging; Fluorescent chemical–genetic hybrids; Semisynthetic fluorescent sensors.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Biosensing Techniques / methods*
Fluorescent Dyes / chemistry*
Humans
Optical Imaging / methods*
Protein Interaction Maps
Proteins / analysis
RNA / analysis

Substances

Fluorescent Dyes
Proteins
RNA