Label-Free Bimetallic In Situ-Grown 3D Nickel-Foam-Supported NH2-MIL-88B(Fe2Co)-MOF-based Impedimetric Immunosensor for the Detection of Cardiac Troponin I

Sathyadevi Palanisamy; Duraisamy Senthil Raja; Boopathi Subramani; Tung-Ho Wu; Yun-Ming Wang

doi:10.1021/acsami.0c09086

Label-Free Bimetallic In Situ-Grown 3D Nickel-Foam-Supported NH₂-MIL-88B(Fe₂Co)-MOF-based Impedimetric Immunosensor for the Detection of Cardiac Troponin I

ACS Appl Mater Interfaces. 2020 Jul 22;12(29):32468-32476. doi: 10.1021/acsami.0c09086. Epub 2020 Jul 8.

Authors

Sathyadevi Palanisamy¹, Duraisamy Senthil Raja², Boopathi Subramani³, Tung-Ho Wu⁴, Yun-Ming Wang^{1

5}

Affiliations

¹ Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-devices(IDS2B), National Chiao Tung University, Hsinchu 300, Taiwan.
² Department of Chemical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan.
³ Department of Food Safety/Hygiene and Risk Management, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan.
⁴ Division of Cardiovascular Surgery, Department of Surgery and Division of Surgical Critical Care, Department of Critical Care Medicine, Veterans General Hospital, Kaohsiung 813, Taiwan.
⁵ Department of Biomedical Science and Environmental Biology, Center for Cancer Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan.

PMID: 32578973
DOI: 10.1021/acsami.0c09086

Abstract

In this study, a simple and competent metal-organic framework (MOF)-based nickel foam (NF)-supported three-dimensional (3D) immunosensor (Ab-NH₂-MIL-88B(Fe₂Co)-MOF/NF) was constructed and utilized for the specific recognition of the biomarker cardiac troponin (I) (cTnI). In the present work, biosensor fabrication was progressed through the modification of the NF substrate with the MOF material (NH₂-MIL-88B(Fe₂Co)-MOF) to enable an amine-functionalized electrode. This amine-functionalized NF electrodes (NH₂-MIL-88B(Fe₂Co)-MOF/NF) were then biointerfaced with anti-cTnI antibodies, which ended up as Ab-NH₂-MIL-88B(Fe₂Co)-MOF/NF electrodes. Analytical executions of the constructed bioelectrode were investigated for the quantitative analysis of cTnI in both buffered and serum solutions. Then, the electrochemical studies were carried out using the electrochemical impedance spectroscopy (EIS) method by monitoring changes concerning the charge transfer resistance (R_ct) characteristics. The limit of detection (LOD) of the Ab-NH₂-MIL-88B(Fe₂Co)-MOF/NF immunosensor was achieved to be 13 fg/mL with great specificity. This kind of immunosensor imparts a new platform for the construction and application of MOF-hybrid 3D electrode materials with enhanced electrochemical behavior in cTnI sensing for the first time.

Keywords: biomarker; cardiac troponin I; immunosensor; metal−organic framework; nickel foam.

MeSH terms

Cobalt / chemistry*
Dielectric Spectroscopy
Electrodes
Humans
Iron / chemistry*
Metal-Organic Frameworks / chemical synthesis
Metal-Organic Frameworks / chemistry*
Nickel / chemistry*
Particle Size
Surface Properties
Troponin I / analysis*

Substances

Metal-Organic Frameworks
Troponin I
Cobalt
Nickel
Iron