Identification of plasmid IncQ1 and NTEKPC-IId harboring bla KPC-2 in isolates from Klebsiella pneumoniae infections in patients from Recife-PE, Brazil

Rev Soc Bras Med Trop. 2020 Jun 22:53:e20190526. doi: 10.1590/0037-8682-0526-2019. eCollection 2020.

Abstract

Introduction: This study investigated the genetic environment of bla KPC-2 in Klebsiella pnemoniae multi-drug resistant clinical isolates.

Methods: Four carbapenemase gene isolates resistant to carbapenems, collected from infected patients from two hospitals in Brazil, were investigated using polymerase chain reaction and plasmid DNA sequencing.

Results: The bla KPC-2 gene was located between ISKpn6 and a resolvase tnpR in the non-Tn4401 element (NTEKPC-IId). It was detected on a plasmid belonging to the IncQ1 group.

Conclusions: To our knowledge, this is the first report of the presence of the bla KPC-2 gene in the NTEKPC-IId element carried by plasmid IncQ1 from infections in Brazil.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • DNA, Bacterial / genetics
  • Drug Resistance, Multiple, Bacterial
  • Humans
  • Klebsiella Infections / microbiology*
  • Klebsiella pneumoniae / enzymology
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / isolation & purification
  • Microbial Sensitivity Tests
  • Plasmids / genetics
  • Polymerase Chain Reaction
  • beta-Lactamases / genetics*

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • beta-Lactamases
  • beta-lactamase KPC-2, Klebsiella pneumoniae