Genetic diversity analysis for narrow-leafed lupin (Lupinus angustifolius L.) by SSR markers

Mol Biol Rep. 2020 Jul;47(7):5215-5224. doi: 10.1007/s11033-020-05596-z. Epub 2020 Jun 23.

Abstract

Narrow-leafed lupin (Lupinus angustifolius L.) is used as grain legumes, fodder for livestock and green manure in the world and has a great potential to be developed as a new crop in China. In this study, we assessed the genetic diversity among a set of 109 newly introduced accessions of narrow-leafed lupin using 76 genomic SSR markers. Data analysis suggested that the average gene diversity index and average polymorphism information content (PIC) were 0.4758 and 0.4328, respectively. The mean allele number per loci (Na) was 6.3816. The population structure analysis identified two subgroups based on delta K (ΔK) values. This result is in accordance with that of a PCA. The AMOVA analysis showed that most of molecular variance were within population. These results will be useful to guide the genetic improvement of the narrow-leafed lupin crop in China.

Keywords: Genetic relationships; Molecular variance; PCA; Populations; Simple sequence repeats.

MeSH terms

  • Genotyping Techniques / methods
  • Genotyping Techniques / standards
  • Lupinus / genetics*
  • Microsatellite Repeats*
  • Plant Breeding / methods
  • Polymorphism, Genetic*