Dietary immature Citrus unshiu alleviates UVB- induced photoaging by suppressing degradation of basement membrane in hairless mice

Erina Tamaru; Mutsuto Watanabe; Yoshihiro Nomura

doi:10.1016/j.heliyon.2020.e04218

Dietary immature Citrus unshiu alleviates UVB- induced photoaging by suppressing degradation of basement membrane in hairless mice

Heliyon. 2020 Jun 15;6(6):e04218. doi: 10.1016/j.heliyon.2020.e04218. eCollection 2020 Jun.

Authors

Erina Tamaru¹, Mutsuto Watanabe¹, Yoshihiro Nomura¹

Affiliation

¹ Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo, 183-8509, Japan.

Abstract

Ultraviolet (UV) irradiation induces physiological and morphological skin damage, resulting in skin dryness, wrinkle formation, and loss of elasticity. The basement membrane (BM) has been shown to play crucial roles in binding epidermis to dermis tightly, regulating cell differentiation and proliferation, and signaling protein production. Dietary flavonoids have been revealed to improve the damage caused by UV exposure. Immature Citrus unshiu is known to contain high concentrations of flavonoids such as hesperidin and narirutin. In this study, the effects of immature Citrus unshiu powder (ICP) on photoaged skin were demonstrated using UVB irradiated hairless mice. Oral administration of ICP improved loss of skin hydration and increase of transepidermal water loss. The histological analyses of hairless mice dorsal skin revealed that oral administration of ICP improved UVB-induced overgrowth of epidermal cell, suppressed epidermal cell mortality and BM destruction. Therefore, the administration of ICP could improve photoaging by protecting the tissues around BM.

Keywords: Aging; Antioxidant; Antioxidants; Basement membrane; Biochemistry; Cell biology; Citrus unshiu; Extracellular matrix; Flavonoids; Food component analysis; Hairless mice; Hesperidin; Laminin; Narirutin; Phenolic compound; Photoaging; Ultraviolet light.