The role of Rhipicephalus sanguineus ticks parasitizing dogs in the spread of tick-borne rickettsial pathogens in the city of Sevastopol

E E Alieva; E I Bondarenko; K D Maliy; A N Shvalov; E A Verbenets; M T Gafarova

doi:10.1016/j.nmni.2020.100704

The role of Rhipicephalus sanguineus ticks parasitizing dogs in the spread of tick-borne rickettsial pathogens in the city of Sevastopol

New Microbes New Infect. 2020 Jun 3:36:100704. doi: 10.1016/j.nmni.2020.100704. eCollection 2020 Jul.

Authors

E E Alieva¹, E I Bondarenko², K D Maliy³, A N Shvalov⁴, E A Verbenets⁵, M T Gafarova³

Affiliations

¹ Federal State Budgetary Institution 'N.I. Pirogov Saki Military Clinical Sanatorium', Ministry of Defence of Russia, Saki, Russia.
² AO 'Vector-Best', Novosibirsk, Russia.
³ S.I. Georgievsky Medical Academy (Academic Unit), V.I. Vernadsky Crimean Federal University, Simferopol, Russia.
⁴ State Research Centre of Virology and Biotechnology 'Vector', Koltsovo, Novosibirsk, Russia.
⁵ Centre for Prevention and Control of HIV/AIDS, Sevastopol, Crimea, Russia.

Abstract

The occurrence of Mediterranean fever with periods of increase and decrease has been recorded in the Crimean peninsula. The city of Sevastopol and its vicinity are known endemic areas for this disease. Some of the most active agents in the spread of this rickettsiosis are feral and abandoned dogs. The aim of this study was to test ticks parasitizing dogs in Sevastopol for the presence of Rickettsia using molecular methods. The testing of ticks was carried out using real-time PCR and the 'Real Best DNA Rickettsia species' kit (AO 'Vector-Best') followed by sequence identification of the rickettsial DNA detected. The DNA marker for Rickettsia species (a conservative area of citrate synthase gene, gltA) was detected in 16 of 84 (19.1%) samples of Rhipicephalus sanguineus ticks tested. Larger fragments of gltA, ompA and sca4 were amplified and sequenced for 10 of 16 PCR-positive samples. Rickettsia DNA amplified from eight of the samples matched the sequence of Rickettsia conorii conorii Malish, the causative agent of Mediterranean fever. The sequences of Rickettsia DNA from two other ticks had the closest match to homologous fragments of Rickettsia massiliae, a pathogenic spotted fever rickettsia that was identified in the Crimean Peninsula for the first time as part of this study. The detection of two pathogenic species of Rickettsia in the studied ticks suggests the potential for two rickettsial diseases in the region and warrants further epidemiological and clinical studies.

Keywords: Mediterranean fever; RT-PCR; Rhipicephalus sanguineus; Rickettsia; Rickettsia conorii; Rickettsia massiliae; Ticks.