Chromobox 2 Expression Predicts Prognosis After Curative Resection of Oesophageal Squamous Cell Carcinoma

Sei Ueda; Mitsuro Kanda; Yusuke Sato; Hayato Baba; Shunsuke Nakamura; Koichi Sawaki; Dai Shimizu; Satoru Motoyama; Tsutomu Fujii; Yasuhiro Kodera; Shuji Nomoto

doi:10.21873/cgp.20198

Chromobox 2 Expression Predicts Prognosis After Curative Resection of Oesophageal Squamous Cell Carcinoma

Cancer Genomics Proteomics. 2020 Jul-Aug;17(4):391-400. doi: 10.21873/cgp.20198.

Authors

Sei Ueda^{1

2

3}, Mitsuro Kanda⁴, Yusuke Sato⁵, Hayato Baba^{1

6}, Shunsuke Nakamura¹, Koichi Sawaki¹, Dai Shimizu¹, Satoru Motoyama⁵, Tsutomu Fujii⁵, Yasuhiro Kodera¹, Shuji Nomoto^{1

3}

Affiliations

¹ Department of Gastroenterological Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
² Department of Maxillofacial Surgery, School of Dentistry, Aichi-gakuin University Graduate School of Medicine, Nagoya, Japan.
³ Department of Surgery, School of Dentistry, Aichi-gakuin University Graduate School of Medicine, Nagoya, Japan.
⁴ Department of Gastroenterological Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan m-kanda@med.nagoya-u.ac.jp.
⁵ Department of Thoracic Surgery, Akita University Graduate School of Medicine, Akita, Japan.
⁶ Department of Surgery and Science, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.

Abstract

Background/aim: To investigate the function of chromobox 2 (CBX2) in oesophageal squamous cell carcinoma (OSCC).

Materials and methods: We used real-time quantitative reverse transcription PCR (qRT-PCR) and immunohistochemistry to determine CBX2 expression levels in 13 human OSCC cell lines and clinical specimens of two independent cohorts of patients with OSCC.

Results: PCR array analysis revealed that CBX2 was co-ordinately expressed with WNT5B in OSCC cell lines. RT-qPCR analysis of clinical samples revealed a high tumour-specific CBX2 expression compared with normal oesophageal tissues. High CBX2 expression was significantly associated with shorter disease-specific survival, hematogenous recurrence, and overall recurrence. Analysis of tissue microarrays of one cohort revealed that patients with higher CBX2 levels tended to have a shorter disease-specific survival.

Conclusion: CBX2 overexpression in OSCC tissues may serve as a novel biomarker for predicting survival and hematogenous recurrence.

Keywords: Biomarker; chromobox 2 (CBX2); oesophageal squamous cell carcinoma; recurrence.

MeSH terms

Aged
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism*
Esophageal Neoplasms / metabolism
Esophageal Neoplasms / pathology*
Esophageal Neoplasms / surgery
Esophageal Squamous Cell Carcinoma / metabolism
Esophageal Squamous Cell Carcinoma / pathology*
Esophageal Squamous Cell Carcinoma / surgery
Esophagectomy / mortality*
Female
Follow-Up Studies
Humans
Male
Neoplasm Recurrence, Local / metabolism
Neoplasm Recurrence, Local / pathology*
Neoplasm Recurrence, Local / surgery
Polycomb Repressive Complex 1 / genetics
Polycomb Repressive Complex 1 / metabolism*
Prognosis
Retrospective Studies
Survival Rate
Tumor Cells, Cultured

Substances

Biomarkers, Tumor
CBX2 protein, human
Polycomb Repressive Complex 1