MicroRNA-4331-5p promotes FMDV replication through inhibiting interferon pathways in PK-15 cells

Virus Res. 2020 Sep:286:198064. doi: 10.1016/j.virusres.2020.198064. Epub 2020 Jun 20.

Abstract

MicroRNAs play vital roles in regulating the battle between pathogens and host cells during viral challenging. MiR-4331 aggravates transmissible gastroenteritis virus (TGEV) -induced mitochondrial damage, also suppresses transcription of TGEV gene 7 via targeting cellular CDCA7. Otherwise, miR-4331-5p affects H1N1/2009 influenza A virus replication by targeting viral HA and NS. However, whether microRNA ssc-miR-4331-5p (miR-4331-5p) regulates foot and mouth virus (FMDV) replication remains unclear. To explore the role of miR-4331-5p in FMDV infection, we detected the expression level of miR-4331-5p in porcine kidney (PK-15) cells. The results showed that FMDV infection directly upregulates miR-4331-5p expression, while transfection of mimics or inhibitor of miR-4331-5p promotes or inhibits FMDV replication. Further investigation clearly showed that miR-4331-5p increases FMDV replication through inhibiting type I interferon pathways. These data demonstrate that miR-4331-5p plays an important role in regulating FMDV replication.

Keywords: FMDV; Interferon; miRNAs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Foot-and-Mouth Disease Virus / genetics
  • Foot-and-Mouth Disease Virus / physiology*
  • Host-Pathogen Interactions* / genetics
  • Host-Pathogen Interactions* / immunology
  • Interferon Type I / antagonists & inhibitors*
  • Interferon Type I / immunology
  • MicroRNAs / genetics*
  • MicroRNAs / immunology
  • Swine
  • Up-Regulation
  • Virus Replication*

Substances

  • Interferon Type I
  • MicroRNAs