Objective: This study aimed to investigate the molecular mechanism of RAB1A in the proliferation, invasion, and metastasis of human tongue squamous cell carcinoma.
Methods: Western blot was used to detect the expression of RAB1A protein in human normal tongue epithelial cells (Hacat) and tongue squamous cell carcinoma Tca8113. The changes in RAB1A after plasmid transfection were also studied. The Tca8113 cells were named SiRAB1A/Tca8113 after RAB1A plasmid transfection. The expression of the epithelial-mesenchymal transition (EMT)-related markers of SiRAB1A/Tca8113 cells was also detected. CCK-8 assay was used to detect the proliferation of SiRAB1A/Tca8113 cells. Transwell and wound healing assays were used to detect the invasive and metastatic abilities of SiRAB1A/Tca8113 cells, respectively.
Results: Western blot results showed that the expression of RAB1A in tongue squamous cell carcinoma cells was significantly higher than that in Hacat. RAB1A decreased significantly after SiRAB1A plasmid transfection. CCK-8 proliferation assay showed that the proliferation of SiRAB1A/Tca8113 cells also decreased significantly. Transwell and wound healing assays demonstrated that the invasive and metastatic abilities of SiRAB1A/Tca8113 cells decreased significantly, respectively. In addition, Western blot results demonstrated that RAB1A deletion significantly increased the expression of E-cadherin and inhibited the expression of Vimentin.
Conclusions: RAB1A could promote the proliferation, invasion, and metastasis of tongue squamous cell carcinoma cells.
目的 探讨RAB1A对人舌鳞状细胞癌细胞增殖、侵袭和转移影响的分子机制。方法 应用Western blot检测RAB1A蛋白在人正常舌上皮细胞Hacat、舌鳞状细胞癌Tca8113细胞、转染敲除RAB1A质粒后SiRAB1A/Tca8113的表达改变；检测SiRAB1A/Tca8113细胞中上皮-间质转化（EMT）相关标志物的变化；分别采用CCK-8、Transwell、伤口愈合实验检测SiRAB1A/Tca8113细胞的增殖、侵袭、转移能力。结果 Western blot显示Tca8113中RAB1A的表达量显著高于Hacat组，SiRAB1A/Tca8113细胞中RAB1A显著降低；CCK-8增殖实验结果显示，SiRAB1A/Tca8113细胞增殖能力明显降低；Transwell实验结果亦显示其侵袭能力明显降低；伤口愈合实验结果显示其转移能力明显降低；Western blot结果显示SiRAB1A/Tca8113中钙黏蛋白（E-cadherin）的表达显著增加，波形蛋白（Vimentin）的表达显著受到了抑制。结论 RAB1A能够促进舌鳞状细胞癌细胞增殖、侵袭和转移。.
Keywords: RAB1A; epithelial-mesenchymal transition; invasion; metastasis; proliferation; tongue squamous cell carcinoma.