Transcriptomic dataset of Mycolicibacterium smegmatis exposed to an imidazo[1,2- b][1,2,4,5]tetrazine

Aleksey A Vatlin; Ksenia M Klimina; Svetlana G Frolova; Valery N Danilenko; Dmitry A Maslov

doi:10.1016/j.dib.2020.105805

Transcriptomic dataset of Mycolicibacterium smegmatis exposed to an imidazo[1,2- b][1,2,4,5]tetrazine

Data Brief. 2020 Jun 2:31:105805. doi: 10.1016/j.dib.2020.105805. eCollection 2020 Aug.

Authors

Aleksey A Vatlin¹, Ksenia M Klimina¹, Svetlana G Frolova^{1

2}, Valery N Danilenko¹, Dmitry A Maslov¹

Affiliations

¹ Vavilov Institute of General Genetics Russian Academy of Sciences, Moscow, 119333, Russia.
² Moscow Institute of Physics and Technology (State University), Dolgoprudny, 141701, Russia.

Abstract

Deciphering the mechanism of action of novel anti-tuberculosis compounds is a key step in the drug development process. We have previously described a number of imidazo[1,2-b][1,2,4,5]tetrazines with a promising activity on Mycobacterium tuberculosis[1]. These compounds had predicted activity as serine‑threonine protein kinase inhibitors, however spontaneous drug resistant Mycolicibacterium smegmatis mc ² 155 (formerly Mycobacterium smegmatis) revealed only the mycobacterial mechanism of resistance to imidazo[1,2-b][1,2,4,5]tetrazines: mutations in MSMEG_1380 gene lead to overexpression of the mmpS5-mmpL5 operon in M. smegmatis, thus providing resistance to imidazo[1,2-b][1,2,4,5]tetrazines via enhanced efflux [2]. Here we report the RNA sequencing data of M. smegmatis mc ² 155 culture treated with one of the imidazo[1,2-b][1,2,4,5]tetrazines for 1.5 h and the untreated culture as a control. The mapped reads showed that a total of 1386 genes are differentially expressed in this experiment. A further analysis of these data can shed light of the mechanism of action of imidazo[1,2-b][1,2,4,5]tetrazines. The data generated by RNA-seq (raw reads) have been deposited to NCBI sequence read archive (SRA) and have been assigned a BioProject accession number PRJNA615922.

Keywords: DEGs; Drug development; Mycolicibacterium smegmatis; RNA sequencing; Transcriptome; Tuberculosis; imidazo[1,2-b][1,2,4,5]tetrazines.