In 2020 Descemet membrane endothelial keratoplasty (DMEK) has become the gold standard in Germany for the treatment of corneal endothelial diseases; however, the widespread use of DMEK was initially limited due to problems with donor preparation and the difficulty of correctly and gently unfolding the endothelial Descemet membrane (EDM) in the anterior chamber. Following the situational donor selection, the safe single-handed donor preparation of the cornea without tearing or even rupturing the EDM, including the indispensable peripheral semicircular orientation marking. Also presented is a step by step atraumatic loading of the glass cartridge, the introduction of the EDM roll into the anterior chamber, and its safe step by step unfolding based on (1) the sequential use of jets of fluid, (2) repeated tapping on the peripheral/central cornea, (3) controlled flattening of the anterior chamber, (4) use of air bubbles of defined size and finally, the fixation of the EDM in correct orientation to the back of the host cornea with a gas bubble. Since every wrong step with DMEK can have far-reaching consequences for patient and surgeon, this step by step pragmatic approach should minimize the incidence of donor tissue damage and failure in patient maneuvers.
Keywords: Donor preparation; Endothelial corneal diseases; Endothelial pathology; Patient maneuvers; Posterior lamellar keratoplasty.